... IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary antibodies...antibody may be binding non-specifically – try optimizing your blocking and washing conditions or consider...-reactivity. Keep in mind that you may need to optimize the conditions and concentrations of all the different...

...that sometimes it is necessary or desirable to control or look at multiple genes at the same time.  The...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...

...same pace and enter the resolving gel at the same time where they are then separated by size. Gradient ...concentrations, blocking buffers, and incubation times. If the vendor includes publications that cited ...   Analyzing the western blot Once visible, it’s time to analyze your protein of interest. Analysis typically...

...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...mentee’s personality and professional needs at any time. In its Pilot Program 2014-15, IMP provided mentorship... monthly progress video conferences and had real-time correspondence via e-mail throughout the entire ...

...silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved, the...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ... manually extracted. This process is tedious and time consuming, as RNAi mutants with a reduced growth...

...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...switching between different colored ligands at different time points.   Figure 3: Single-molecule tracking...

...of protein function. In addition, the level and timing of expression can also be affected by the tag.  ...immune complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...health and limit debris, keep samples on ice at all times and use gentle pipetting instead of vortexing. Include...

...a large majority of neuronal cells are labeled overtime due to the continued replication of the virus....have been widely used by neuroscientists for some time due to their advantageous characteristics for brain... titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5, AAV8, ...

...It’s a very thankful time of year for those of us at Addgene! And we can’t think of any better way to...Addgene blog? We'd love to hear from you! Email us anytime at [email protected]. ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ....G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses the VSV-...

... have amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and... and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations with...

...world. In the years since, our scientists have optimized many steps in the viral production and quality...virus-related protocol videos that may help to save you time and improve your experiment results! Subscribe to...

...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....up your pipettes, and seal up those plates – it’s time to turn your attention to decorating! To join the...

...Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction 1:01-9:04 : What...We'd like to thank Professor Cepko for taking the time to talk to us and look forward to hear about more...

...to find published plasmids? Do you spend a lot of time in PubMed searching for research articles? Well ...list” to the left of your screen. That’s it! Next time you log-in to your PubMed account and are browsing...

...of gene drives. Listen here! Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast...outreach. Many thanks to Alex Chavez for taking the time to sit down and talk with us.  Alejandro (Alex) ...

... The team with the best costume takes home the ultimate prize – a glorious trophy. Sure it’s plastic and... changing seasons – and they also invited Father Time to the party. They won over our judges with this...

...service and is ready to use out of the box. We estimate that providing cloning grade DNA could save scientists...you want to do with your plasmid without spending time and resources in preparative experiments.  We’re...

...download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has...of requested plasmids, or trends of requests over time. Visualizing the data So, after downloading my plasmid...

...read the following Top 10 posts of 2014, take some time and see what you’re missing. Let us know if you ... Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By...

...modify the expression of multiple genes at the same time.” For more details on how to apply the new system...for CRISPR Genome Editing CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable...

...collaborative approach eliminates redundant work, saving time and allowing precious research dollars to go further... would be most valuable from Addgene during this time. Your input will directly shape our response initiatives...

...MTA), which streamlines the process and saves you time. Anyone affiliated with the recipient organization...number that you entered during checkout. At this time, your organization will have net 30 days to complete...

...native protein. Since this process takes a lot of time and energy, Martí Aldea and collaborators have created... will even replace our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et...

...gotten, consider these headlines in The New York Times and Forbes respectively: "A Powerful New Way to ...Emory University summed it up well in that New York Times feature when he said, “All of this has basically...

...Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible...Now, they’ve developed another AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This...

...in the News (SITN), we recently sponsored a first-time event called DayCon. DayCon is a one-day conference...variety of short- and long-form written content about timely scientific topics, as well as archived seminar ...

...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...of neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can ...

...gain access to an internship on the journey to full-time employment. I have always been interested in the...relating to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s...

...find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors CRISPR toolkit...rhamnose-inducible promoter. While this system was optimized for Burkholderia, they identified 67 genera of...