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Showing: 541 - 570 of 836 results

  1. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    .... Check out Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks...complaining. They may worry that it seems like "tattling" if others are involved. They may feel they are...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    ... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

  5. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...wanted to ensure applicants understood what the expectations were for the intended position. After submitting...Addgene 101” series, where each team gives a presentation on what they do and how it relates to other ...

  6. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    .... The Patriarchi Lab aimed to overcome these limitations by developing and depositing two new norepinephrine... using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing...

  8. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...clear and attractive reports, being good at presentations and of course, networking and collaborating....

  10. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...drop off the box at any location where the United States Postal Service picks up mail. NEB actually started...drop off the box at any location where the United States Postal Service picks up mail. Recycle plastic...

  11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the... the “rabbit hole” of related cell lines when annotating a cell line Community submissions via email: ...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally... experimental setup? Consider what kind of instrumentation you have available, and the conditions of your...

  16. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  18. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  19. Hot Plasmids - October 2022

    Type
    Blog Post
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents, and...

  20. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  21. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

  22. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

  23. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...

  26. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ...

  27. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...extract.    Graphical Abstract: Schematic representation of cell-free system, capable of producing fluorescent...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

  29. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... This tag has been used successfully for immunoprecipitation, western blot, and immunofluorescence with...has the flexibility of also being used for immunoprecipitation, purification, and western blot as monoclonal...
Showing: 541 - 570 of 836 results