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We narrowed to 1,025 results for: URE

Showing: 541 - 570 of 1025 results

  1. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...strand breaks at target sites. Read the paper in Nature Communications Find the Cas12b plasmids  Plant...AAV toolkit - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system...highly customizable as vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read...transferred via conjugation.   Read the paper in Nature Microbiology Find the Mobile-CRISPRi plasmids...

  2. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...launched the cloning grade DNA service. This service features 210 plasmids: empty backbones, fluorescent protein...plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post. 9. Droplet...stably maintain them in the cell. This blog post features two mechanisms that cause plasmids to be incompatible...

  3. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...narrowly focused lectures, interactive panels, and a student poster session. All DayCon lecture content is ...variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs. Their website... engaged. The day kicked off with introductory lectures targeting three broad concepts: the genetic basis... what life is like as a student researcher. The future of DayCon DayCon and other similar events show ...

  4. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...perform transformations. A typical 200mL bacterial culture produces 20mL of competent cells, enough for 800...bacteria, the bacteria is typically grown in liquid culture without antibiotics to give the antibiotic resistance...plate your cells without adding plasmid) to make sure you don’t have contamination.  Also conduct a ...that transforms well in other competent cells) to ensure that your cells can be readily transformed. Compare...previous batches of homemade competent cells to ensure good efficiency.  Want even more tips for transforming...

  5. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...really made me think about my future. They made me rethink having a structured career because our mindsets...how to translate them into real world careers. I figured this internship would be a perfect way of exploring...different experience, It was helpful to have Addgenies reassure me that someone would always be there to help ...experience at Addgene, I am excited to see what my future looks like. ...

  6. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... toolkit SECURE adenine base editor Earlier this year, Keith Joung’s lab developed SECURE cytosine base...compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...RNA-recognition capabilities.  Read the paper in Nature Biotechnology Find the base editors   Single base...editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian cells. ...

  7. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...et al., 2024).   Figure 1: Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024...sequential knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038...cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038...in vivo screening of genes in the immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...climb upwards until they reach a point where the temperature and humidity favors fungal growth. The ants bites...out of the ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the...Vampirovibrio chlorellavorus will not grown in culture alone and relies on its algae host for reproduction...through its gills. It attaches there until they mature and changes sex from male to female. Then, it detaches...resources on the Addgene blog Read about company culture at Addgene See some of our previous Halloween costume...

  9. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ... on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace has...personal development at the center of her lab (pictured above). DePace holds yearly planning meetings ...trainee to create a supportive, growth-oriented lab culture. The aim of these meetings is to give honest, constructive...PI-trainee communication builds a positive lab culture Although they may take some getting used to, yearly... lab culture...

  10. Science as Art

    Type
    Blog Post
    ...screens, but not to know even the life cycle of the creature they deal with.” So photography can be used as...2008. His work exposes and explores the hidden structures within flowers with the use of x-rays. His haunting...and have gathered much acclaim - including being featured in the National Geographic and Smithsonian Magazine...scientist with a new method to explore the inner structures of delicate plants and other organisms without...interest for its stunning images that is has been featured in Time magazine . Other smaller competitions ...

  11. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR. A, Structural.... Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et al., 2023...enhanced response time allows for more precise measurement of norepinephrine uptake throughout the brain...indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038...ColabFold: Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...They generated these editors using the BE4max architecture. The new editors reported in this study have... levels relative to BE4max.  Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi...CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells for...Enhanced CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout... methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you...

  13. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...It was immediately clear that Addgene wanted to ensure applicants understood what the expectations were...responsible for helping other scientists on the team ensure the deposits we received are what the depositing...for me at Addgene. There is a clear effort to make sure people feel connected.    As I became accustomed...aided by the efforts being put in by Addgene to ensure their employees are feeling comfortable.     Lucie... Tips for Writing a Good Cover Letter 7 Tips to Secure a STEAM Internship This Summer  ...

  14. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...called “self-service.” The idea here is to build features that allow users to help themselves, saving time... will continually update the Help Center to make sure that, whatever your support needs are, we’ve got...to the Help Center, you may have noticed a new feature on our website; towards the bottom right corner...www.addgene.org. We’re excited to make these new features available to the Addgene community, and we’d love...Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources at Addgene Visit...

  15. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...was delighted to be invited to the ceremony for surely what is one of many awards that will be bestowed...in markers on a whiteboard. The final image is pictured here…she started from a blank white slate and ...experimentation to the eventual translation to the clinic and cures. There is no doubt that companies are swarming to...computer to fight the known viruses. Bacteria have figured out how to download a bit of virus sequence to ...example, the technology is already available today to cure sickle cell anemia in stem cells but this can’t ...

  16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...facilitating recovery of edited animals. Figure 1: In the background are 504 restriction digests... demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches...making screening for knock-ins much more efficient. Pure PCR repair templates are critical for efficient ...editing efficiency improved by using more dilute mixtures (Doksin et al., 2018). The Mello lab also reported...visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to note that recipients...and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89...

  17. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...discoveries, the world of academia often emphasizes structured methodologies and rigorous research plans. However...strategy could entail immersing yourself in diverse literature. By delving into new topics and expanding your...taking field trips, and spending time outdoors in nature or visiting museums can be excellent methods to... times (or use the notes app on your phone) to capture fleeting thoughts and connections that emerge during...recognizes marvel in all things.   Conclusion  In the structured world of lab research and PhD studies, where ...

  18. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...molecular biology toolbox organized, today’s post features a list of free online molecular biology tools ...provides more tools for plasmid mapping and design (Figure 1). Benchling: While you might think of Benchling...Wayne Davis from the University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame...tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates a sequence...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...

  19. Fostering communication in remote collaborations

    Type
    Blog Post
    ...can be tricky, and it takes extra effort to make sure everyone in the meeting has an equal opportunity...emailing documents to one person at a time. Just be sure there is a point person responsible for incorporating...will come from the collaboration. Some things to figure out are timelines for experiments and manuscripts...from each other, access specialized equipment, or ensure that techniques are being performed the same way...feedback with everyone involved can speed up the future directions of the project. The state of research...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...invading phages but to also destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins... immune system seems like the winning ticket to ensure prokaryotic survival; however, viruses, too, are...strongest will to survive. Genome engineering and future directions CRISPR/Cas inspired genome editing was..., Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy...Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L, Ran FA, Cox ...

  21. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...because it turns a dynamic process into a static measurement and it provides limited information about what...fluorescent autophagy reporters now allows for the measurement of autophagic flux, or the changes in autophagic...strength excited at 586 nm to 440 nm serves as a measure of autophagy induction: when Keima is in the cytosol...is high. When untargeted, Keima can be used to measure bulk autophagy, but the addition of a mitochondria-targeting...18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy in mammalian...

  22. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...2010). Rather than being released into the cell culture supernatant, the viral particles associate with.... Coroadinha, A. S., et al. "Effect of osmotic pressure on the production of retroviral vectors: Enhancement...overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering...Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but ...serotype-dependent release of functional vector into the culture medium during adeno-associated virus manufacturing...

  23. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...we've been able to measure their relative ability to neutralize.”   Figure 2: Sera used in the...was not tested at the time of this study. Figure 1: Neutralization levels against spike pseudovirus...mutations was surprising,” says Balazs.   Figure 3: Neutralization outcomes of convalescent sera....V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021...

  24. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...🎶 “Bye bye L-arabinose drivePut your cultures in the shakerTurn the LEDs onAnd when you wantYou can ... a light-responsive domain that dimerizes upon exposure to blue light (Fig. 1). Another blue light-inducible...light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional BLADE variants, too...expression needed for most applications.   Figure 1: With blue light, the VVD domains of the BLADE...how spatially precise the activation is.   Figure 2: A bacteriograph of Michelangelo's "The Creation...

  25. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ... lines (hybridomas) that secrete antibodies in culture and provide renewable reagents with reliable and...plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition, and...ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies using quantitative...Reproducibility: Standardize antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury... (2015) Antibodies: validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury...

  26. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...introduction, and a resolution.   Fig. 2: The structure of a story - not just for fiction! Image credit...more. This could be by outright stating the big-picture problem, by asking your audience a simple question...’t need to be anything fancy; it just needs to capture your audience’s attention enough to make them decide... new questions are next or how this will impact future research or policy.  It is worth mentioning that...patients or other affected people, man/animal vs nature, anthropomorphized animals, or even seemingly unrelated...

  27. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...CPP-tagged protein and an untagged control could enter cultured cells and activate expression of a luciferase ...After iTOP treatment, both primary (hESCs) and cultured cells showed up to 70% resistance to diphtheria...enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances...elsewhere in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or...Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic ...

  28. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...abilities, or other phenotypes that can be efficiently measured at the single cell level), are separated by, for...use ~400X more cells than gRNAs in a screen, to ensure that each gRNA has a good chance to contribute ...can be used: Design a screen with an easily measureable cellular phenotype (e.g. survival of a cancer...and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed PMID...and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903... parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826. PubMed PMID: 26167643...

  29. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... susceptibility of biofilms formed on various structured surfaces. Plasmids expressing fluorescent proteins...deletions on biofilm formation and growth will then be measured to link the deleted genes to specific functions...several diseases. Her goal is to identify and isolate pure plant compounds with antidiabetic properties that...river, take water samples at specific locations, measure fluorescent signals from the sensor, and send a.... Subscribe to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees...

  30. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...research group in Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership...cutting-edge research, is still one of the most valuable features of modern science in our view. And it was this...that prompted us to create Crowdfight.   Figure 1: Crowdfight is a community of scientists who ...collaboration and join efforts to shift scientific culture towards a more generous and rewarding way of making...receiving dozens of requests every day.    Figure 2: When a researcher needs a collaborator for a...
Showing: 541 - 570 of 1025 results