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Showing: 721 - 760 of 1100 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...thus tracking the localization of that biomolecule inside living cells. FPs classified by the emission color...range, there are other traits that need to be considered when choosing an FP: Unique categories of fluorescent...distance between the two FPs is <10nm, and should be considered when labeling proteins that interact. Indeed,... coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared...greenfluorescentblog.wordpress.com.   More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent...

  2. MXS Chaining

    Type
    Blog Post
    ... degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation... can be assembled. For example, because CpG dinucleotides are underrepresented in most vertebrate genomes...and MluI recognition sites, which contain CpG dinucleotides in their 6-mer sequences, are rarely found in...for cloning genes from organisms with high CpG dinucleotide representation. The specific restriction enzymes...

  3. An Introduction to Adenovirus

    Type
    Blog Post
    ...Heinrich, W. (1983). Worldwide epidemiology of human adenovirus infections. Am J Epidemiol, 117(4), 455–466...length. Human adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting...adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679–1687. https...

  4. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...After learning of it's continued popularity, I decided this protocol would be a worthy first contribution...protocols. After my introduction to protocols.io, I decided to research what other online (and mobile) tools...www.addgene.org/ If you’re working with plasmids and you decide to share them using Addgene’s repository, you can...Protocols OpenWetWare.org (OWW) is a wiki that provides a community for biologists and bioengineers to...and allows scientists to contribute protocols or provide links to protocols on their own websites. We're...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... but many find it helpful to obtain support and ideas from those who seem to be managing. Being reminded...balance tips, we need to look to for support and ideas to manage our stress. If you are saying to yourself...appreciate this – and they will be happy too. On the flip side, if you are not happy at work, you probably need... you will get help when you need it. On the work side, join a professional community that you can count...an emergency and this will make you much more confident about their safety when they leave home which ...

  6. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Mortensen et al., 2019). Our method might provide some useful guidelines for the development of transient transformation...transformation systems for other species as well. Things to consider in developing a transient leaf transformation ... promoter in Agrobacterium. This can be easily avoided by the use of introns, which will be removed from... to quickly assemble vectors and test different ideas. We are a huge fan of the Golden Gate based MoClo...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...that evolved in the last 10,000 years (which is considered recent). They have diverse traits with good genetic...control who a female wants to mate with and if she decides to raise their offspring or leave them to their...a cool fluorescent indicator system to rapidly identify recombinants. Arthropods (Jason Rasgon) This lab...

  8. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...Hedgehog but this is not a direct reference to the video game character but rather to an extremely important...Christine Nusslein-Volhard and Eric Wiechaus first identified the hedgehog gene in Drosophila. They observed...nonsyndromic holoprosencephaly where the brain fails to divide properly into the right and left hemisphere. Addgene...origins of the name but some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers...Additional resources on the Addgene blog Read our Quick Guide to Working with Drosophila Read our Plasmids 101...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ... you have these rules (word limit, formatting guidelines, etc.) you have some criteria to shape the document...research papers that I needed to read. Once I had an idea of the themes in the field, I searched for recent...field. I made sure to find information from genome-wide studies, as well as results from smaller and more... or popular papers, but looked for papers from a wide range of authors. Just start writing When I first...Plasmids Review the CRISPR Field with Our CRISPR Guide Pages ...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than... monitored throughout production, ensuring the identity of each sample from the DNA tube all the way to... preparation. After production, we confirm the identity of the transfer plasmid in the viral preparation...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...ternary complex with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2...important functional properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024...CRBNmidi as a promising tool for future structure-guided drug design and studies of protein ubiquitination...showed greater photostability and also matured considerably faster, with expression levels comparable to...expression and removing the sgRNA plasmid after identifying successful knockouts limits further cutting and...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...negative staining. Empty vector particles can be identified after negative staining and appear darker than...been used to titrate AAV, but this method is not widely used today (Fagone et al., 2012). PCR-based methods...from different sources in the same experiment, consider retitering both AAV in your lab. The absolute ...does not give an indication of infectious titer, consider titrating each lot of AAV in vivo to determine...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... one bacterium sharing genes with another, an accidental transmission of genes from one bacterium to another...tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance on...different reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment... antibiotics and which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type of RNA transcript...transcript     Description Expression Additional considerations CMV General expression mRNA Strong mammalian... phosphoglycerate kinase gene.  Constitutive  Widespread expression, but may vary by cell type. Tends ... used for Description Expression Additional considerations T7 in vitro transcription/ general expression...therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters as described...

  15. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    ...Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics Guide  ... our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful resource...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary to their target sequence...plasmid and call it a day. You need to include both sides of the complementary RNA duplex and the hairpin ...169)","rgba":"rgba(0, 82, 169, 0.75)"},"override_default_style":true,"text_hover_color":{"color":"#ffffff...time you need to knock down a gene, maybe you’ll consider using RNAi! References and Resources References... R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), ...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...so we’ve been busy updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers...week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not into Twitter, we have some exciting...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...other methods below should help in multimer identification.   Fig. 2: Gel image of Plasmid 61564...used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services using long-read...within a plasmid and, therefore, allow for the identification of plasmid multimers. Capillary Gel Electrophoresis...laser-induced fluorescence (CGE-LIF) for detection and can provide data on the quantity of various topologies and ...

  19. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...this concept in his microbiology lab course, he decided to add a bit of fun. He mixed together 10 E. coli...host strains affect transformation efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course...plasmids here, and check out our streak plate protocol video on Youtube:     Additional resources on the...plasmids at Addgene Check out additional written and video protocols ...

  20. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...insights into the process of getting an MD PhD, the inside scoop on developing new CRISPR tools, and the potential... : Alex introduces himself and explains why he decided to do an MD PhD - it's all in the family. 3:56 ...Biologically Inspired Engineering and why Alex decided to work there. 13:22 - 22:07 : Alex's projects ... Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn ...

  21. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ... In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...compatible with the introduction of unique molecular identifiers, which can improve the statistical power of in... of the pRDA_526 vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses...Context-Dependent Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System...

  22. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...some good guidelines to follow in order to ensure that people can quickly and easily identify what your...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

  23. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...citations for older publications. (As an interesting aside, Nature recently reported that the most cited work...their own libraries and offering another way to consider and quantify the impact of scientific publications...find ways to capture scholarly work that exists outside of traditional scientific journals. Of course, ...

  24. Science Communication for Everyone

    Type
    Blog Post
    ...communication? It is important to identify your audience and consider their background. Social media is...contribution can either be an article, a short post, a video, or a piece of sci-art.  What are your top tips ...communication posts Sign up to receive our science career guide Learn about different careers in science communication...

  25. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

  26. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...puzzle fell into place, as they, taken alongside the insights provided by the studies in Tsien lab, covered...victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of A. victoria there is a ring...1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein could be used...

  27. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit...fixable dye stains that react with free amines on and inside the cells and are thus typically used for distinguishing...vector.) In our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction...should exclude APC-Cy7 from the rest of our panel. Besides some background cell death constantly happening...Compensation Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository...

  28. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...antibody, which recognizes the primary antibody and provides the method of detection. You’ll also want to use...is used to detect proteins on the surface of or inside whole cells. Antibodies - typically, primary antibodies...staining. This panel of antibodies is used to both identify the cell subsets of interest from among the mixture...This could be cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture...IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978...

  29. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    .... Although my interests are very clear, I had no idea how to translate them into real world careers. I...this new virtual world, I was worried about how my ideas would be translated through Zoom calls and Google...frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for what my Instagram...with Quintin Marcelino who is Addgene’s Science Video Production Specialist and Arthur Guerra who is a...

  30. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...Cre - A useful tool for neuroscientists AAVs are widely used by neuroscientists because they efficiently...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. For example...only be expressed in neurons. Indeed, this AAV provides you with a means to specifically activate expression... viral vector blog posts for many more tips and ideas on how to use these fantastic tools in your experiments...

  31. Editor's Choice, September 2016

    Type
    Blog Post
    ...Stacey from Eastern Tennessee State University and provides a brief rundown of Sean’s top five smartphone ... lab life a bit easier. Since the post doesn’t provide an exhaustive list of all the apps out there, we...reasons: 1) I think phage are super cool and have considered getting a tattoo of my favorite molecular biology...to screen for proteins that could be useful for a wide variety of reasons. 3) I would love to see more ...

  32. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... but if it is not possible consider a phone or video call. If you do decide to use Skype, make sure to... from other scientists doing interesting work. Consider this to be like any other research project. You...

  33. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open science, ensure ...MOOC), with an explosion of courses and content providers and participation from many well-respected and...

  34. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...them remain uncharacterized. This represents a considerable barrier to furthering research and developing... an assortment of genes. Using patient data to identify mutations of unknown importance in cancer Previously... Center patient databases, this research team identified 1,049 perturbations in several genes spanning... 33 cancer types. The types of mutations they identified included gene amplifications and fusions, missense...

  35. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Maureen Hanson’s and Rima Menassa's labs made the accidental discovery that laser treatment can photoconvert... Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible fluorescent...great scientific discoveries, this one was made by accident. After exciting GFP-labeled plant cells at 405...variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent proteins for your imaging...

  36. Science as Art

    Type
    Blog Post
    ...spoke with Daniel about the interplay of these two ideas he said “science is a form of art to me… The problem...to have increased value and is appreciated by a wider audience for more than just its biological importance...of familiar flowers." This new x-ray technique provides scientist with a new method to explore the inner...organisms without destroying the original, which could provide a new tool to study old and unique samples, such...

  37. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...succesful scientists have time for fun and life outside of the lab? This final post in the PI Interview...other. The Church Lab enjoys each other’s company outside of the lab by sharing meals, going on trips, and...school year winds down, take some time to have fun outside the lab! How does your lab celebrate each other's... fun outside the lab...

  38. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...base editor, BE3, induces a low frequency of genome-wide off-target DNA editing. Using several rapid and ...-effective screening methods, David Liu’s lab identified and validated CBEs with reduced Cas9-independent... tool from Albert Cheng’s lab that enhances RNA-guided methylcytosine editing by TET1 by co-delivering...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

  39. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university...anti-tech or pragmatic.” Advice for pursuing careers outside of academia Recently, graduate students and postdocs...skills that are applicable to a variety of careers outside of academia. She has had previous graduate students...

  40. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty quickly notice...fluorescent proteins to observe what’s happening inside cells for at least a couple of decades. Green is...jellyfishes and corals, but all of them fluoresce outside the transparency window of mammalian tissues,” ...capable of revealing, for example, how muscles work inside a living animal. For those making use of these ...
Showing: 721 - 760 of 1100 results