We narrowed to 795 results for: Tec

1. A Look at Addgene's QC Process

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   Blog Post

   ...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...

2. Making CRISPR Plasmids Using Fragmid

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   ... enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org...

3. Progress Towards a PAM-Free CRISPR

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   ...genomic landscapes should be editable with CRISPR technology.     Fig. 1: SpCas9 has sparse PAM coverage...

4. Viral Vectors 101: Calcium Sensors

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   ...jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods Many calcium reporters use a single fluorescent...

5. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest to MoClo ...

6. Viral Vectors 101: Chemogenetics

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   ...Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth...

7. Hot Plasmids and Viral Preps - March 2021

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   ...AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of FPs as well...

8. Lab Automation at Addgene

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   ...Find protocols for standard molecular biology techniques Browse our video library Check out our molecular...

9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...more about precise neuronal targeting using AAV technologies Browse all of our viral vector blog posts Resources...

10. Early Career Researcher Toolbox: Finding Relevant Papers

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    ...papers, and more. Saving papers There isn’t technically a way to save papers on Twitter, but you can ...

11. Overcoming the Challenges of Lentiviral Production

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    ... packaging system has become available through Clontech. This system improves safety by dividing the viral...

12. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...and not targeted to a specific area. Some other technical considerations for stereotaxic injections are:...

13. Open Resources and Plasmid Tools For Studying C. elegans

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    ...keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression and ...

14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications Specialist at AsisChem Inc. and...

15. Tips for Titering Your Lentiviral Preps

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    ...of lentiviral vector titration methods."BMC biotechnology 6.1 (2006): 1. PubMed PMID: 16836756. PubMed...

16. The Materials Science of Optogenetics Experiments

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    ...flexibility in behavioral experiments. However, the technical demands of using these wireless devices are significantly...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ...Postdoctoral scholars should have the skills and techniques needed to facilitate effective teamwork, manage...

18. The AAVantages of AAV in CRISPR Screens

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    ... development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily...

19. Production of Virus in Insect Versus Mammalian Cells

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    ...viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991...

20. Antibodies 101: Flow Compensation

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    ... immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School...

21. Plasmids 101: Degron Tags

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    ...Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun 13...

22. PhD Applications After COVID

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    ...more difficult. I’ve learned that some of the technologies that helped us navigate a global pandemic can...

23. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...goes into a 96-well plate and comes out of it. Biotech companies are known to have tools that do this ...

24. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304....

25. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

26. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...newly tagged gene. Most insertions can only be detected by labor-intensive processes like PCR or by evaluating...

27. The Scientific Conference Poster Session: Tips for Success

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    ...lab mates, and PI can provide feedback on your technical presentation, but those outside of your field ...

28. Anatomy of a Plasmid Page at Addgene

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    ...are distributed under the terms of the UBMTA to protect our depositors’ IP, but there may also be extra...

29. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...new tools for plant science based around those technologies in the near future. Science on Twitter Addgene...

30. Great Results Start with Great Standard Curves

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    ...series of the standard and the minimum and maximum detection limits of the instrument being used to measure...

31. Viral Vectors 101: The Retroviral Lifecycle

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    ...Indoor and Outdoor Air. Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT...

32. Tips for arabidopsis transformation

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    ...Transgenic Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi...

33. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...DNA-templated synthesis are four examples of technologies pioneered in his laboratory. Learn more here...

34. DNA Purification Without a Kit

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    .../ul) could still be eluted from the column, as detected by qPCR, but this did not reduce cloning efficiencies...

35. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...results at all, let us know! Contact our scientific technical support at [email protected] and let us help.  ...

36. Summer SciComm Series: Modes of Communication

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    ...internet access. High probability of real-time technical difficulties Exhibits Medium to high Audience...

37. Lentiviral Vector Uses and Overview

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    ...transgene expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in ...

38. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...Every step along the way involves good aseptic technique and controls to ensure that nothing is contaminated...

39. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...J., & Fan, M. (2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

40. Plasmids 101: Stringent Regulation of Replication

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    ...Bacteria. In Fundamental Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano...