We narrowed to 990 results for: Abo

...light-regulated two component systems designed by the Tabor lab. Which inducible promoter is right for me? Here...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...

... view of traces corresponding to the dashed box above. The number of action potential (AP) spiking events...

...you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information...

...all be important.  If we eliminate the problems above: unclear methods, QRPs, and small samples, then ...

...to high copy numbers. This creates a potential metabolic burden on the yeast cells. Some phenotypes may...

...Addgene Blog Find microbiology blog posts Read about synthetic biology on the blog Find all of our Plasmids...

...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...

...degrade any protein. We are particularly excited about the dTAG system as we can use it to evaluate the...

...articles from April 2018. If you'd ever like to write about a recent plasmid deposit please sign up here. No...

...Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins Control Protein...

...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

...Additional Resources on the Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the...

...indirect approach - which means it’s time to talk about secondary antibodies. While a primary antibody ...

...that this blog has answered all of your questions about polyhistidine tags. For more antibody-related information...

...How Barcodes Can Be Used in Lineage Tracing Read about CRISPR/Optogentics Tools Resources on Addgene.org...

...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...

...results, and subsequent conclusions. Being vigilant about these errors is an important part of maintaining...

... to devise an experiment that can tell you more about its function.  Resources and references Resources...

...completely inert, but others can cause diseases. Nervous about handling viruses? That’s okay! In this article we...

...In a direct approach, that is simple - but what about when you need to use an indirect (primary and secondary...

...drawback of this approach is that it can be quite labor intensive. Target cells are transduced with serial...

...Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch Up On Your...

...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

...dehydration (Howard & Harvey, 2017). You can learn more about common AAV applications by reading our Viral Vectors...

... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

... Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid ...

...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

...dependencies associated with each ancestry group. Since about 90% of the cell lines used in DepMap CRISPR screens...

...asked why they don’t share, many express concern about sharing negative data they cannot explain, perhaps...

...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....

...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

...University of Tokyo, Osaka University). Can you speak about your different experiences in research labs. What...

...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

...Collection and the BRAIN Armamentarium Collection, a collaborative effort that the Allen Institute contributes ...

...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...