We narrowed to 1,023 results for: CAT;

1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   ... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

2. Sensing Neuronal Dopamine

   Type

   Blog Post

   ...at a certain frequency, and this frequency can indicate behavioral state, for example, if someone is awake...

3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   ...depolarized and will fire a signal. The non-specific cation channel ChR2 and the calcium channel LiGluR both...

4. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   ...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...

5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

   ... curation, technical service and open-access educational resources. What is a nonprofit? The term "nonprofit...

6. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   ...featured 20 mutations in TnsB, the subunit that catalyzes DNA insertion, along with both evolved and engineered...

7. New Viral Vectors - Fall 2024

   Type

   Blog Post

   ...vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 ...

8. An Addgene Summer Internship

   Type

   Blog Post

   ...compiled a list of three options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528...

9. Antibodies 101: Fc Effector Functions

   Type

   Blog Post

   ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in...

10. Build Your CRISPR Vocabulary

    Type

    Blog Post

    ...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

11. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    ...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...

12. Running for Rare Disease, Running for FOP, Running for AJ

    Type

    Blog Post

    ...collecting donations for IFOPA, an organization dedicated to raising funds and awareness of FOP. Please ...

13. Management for Scientists: Managing vs. Leading

    Type

    Blog Post

    ...in the way of the doers and by ensuring good communication. A manager must both hire and fire to make sure...

14. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    ...scientists discussed new CRISPR delivery systems, modifications to the RNA components, and challenging target...

15. Management for Scientists: What Makes a Good Manager Anyway?

    Type

    Blog Post

    ...Like any other skill, it can be developed with education and practice. Second, it takes a focus on the ...

16. Meet the New Addgene Board Members!

    Type

    Blog Post

    ... interference. In the first two months since publication, the lab got over 100 requests for the plasmids...

17. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    ...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme...

18. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...

19. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    ... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...

20. New Viral Vectors - Winter 2025

    Type

    Blog Post

    ...vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2...

21. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type

    Blog Post

    ...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...

22. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    ...there a move with either promotions or further education may allow you to transition into various realms...

23. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type

    Blog Post

    ...session to another,” Bachle says. “Now, the physical location didn’t matter. I didn’t have to consider that ...

24. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    ... Protocols for Work with Virus Watch Our AAV Purification Video ...

25. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    ...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...

26. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    ...availability date or to be notified when a particular catalog item is available for order please email [email protected]...

27. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    ...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...

28. Four Factors that Differentiate the Stem Cell Field

    Type

    Blog Post

    ... have profound impacts on the development of applications with the potential to save lives. While most...

29. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    ...they might be able to do and to organize it and catalogue it, I mean that’s the critical function really...

30. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

31. Advice for Scientists Starting a Lab

    Type

    Blog Post

    ...environment for research is totally dependent on communication amongst the team so getting everyone talking...

32. Finding and Joining Your Dream Lab

    Type

    Blog Post

    ... next.” Connie Cepko looks for people who are dedicated and interested in science. “Postdoc hires are ...

33. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type

    Blog Post

    ... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

34. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    ...availability date or to be notified when a particular catalog item is available for order please email [email protected]...

35. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ...prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein...

36. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    ...compensation.    Working with antibodies can get a bit complicated, but hopefully this helps you successfully select...

37. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    ...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow...

38. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    ... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

39. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    ...In fact, selections are often inappropriately categorized as screens! Why the confusion? The commonality...

40. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    ...interactions of proteins within these structures. Enzyme-catalyzed proximity labeling (PL) uses a promiscuous labeling...

41. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ...Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous...

42. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...This is a chance to practice leadership and communication skills. The leader also provides materials such...

43. dTAG - You're it!

    Type

    Blog Post

    ... and we will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG...

44. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

45. Antibodies 101: Fab Fragments

    Type

    Blog Post

    ...fragments described above can be used for similar applications and have similar advantages, though with some...

46. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...

47. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor...

48. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

49. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

50. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...