We narrowed to 988 results for: IGH@

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

...community through sharing science and distributing high-quality materials and information. As a scientist...

...lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR...

...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

... Find job listings at Addgene Get more career insight from Addgenies ...

...One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (...

...little more certain that I was being led down the right path. The frequent Zoom meetings helped me communicate...

...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

...expression system. If you find that your protein might not fold well, you should screen a few different...

...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...

...looking at their performance in categories such as brightness, stability, and disruption of the tagged protein...

...be targeted with optical stimuli. The restricted light exposure to a specific area in combination with ...

... Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination settings...

...pinpoint exactly when I realized I had made the right decision to choose Addgene for my co-op, but I know...

...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...’s not our only job - we strive to also give you high-quality educational resources to help you do better...

...document may sound like something out of your nightmares, shared ownership of materials produced from ...

...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown...

...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...

... the same protein, their gene sequence may have slight or large variations depending on how closely related...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

...Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...

... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...

...back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even...

... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...

...business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our...

... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...

...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...

... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...

...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...

...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...

...is important to know that the student presenting right before me had been assigned a structure paper with...