We narrowed to 431 results for: {{{s}}}

1. Bioinformatics at Addgene

   Type

   Blog Post

   ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...

2. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

3. Great Results Start with Great Standard Curves

   Type

   Blog Post

   ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

4. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   ... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

5. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

6. Getting to Know Expansion Microscopy

   Type

   Blog Post

   ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

7. DNA Purification Without a Kit

   Type

   Blog Post

   ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...

8. Hot Plasmids - November 2023

   Type

   Blog Post

   ... Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein...

9. How to Be an Excellent Trainee

   Type

   Blog Post

   ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

10. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...

11. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

12. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

13. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

14. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

16. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell...

18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

20. MXS Chaining

    Type

    Blog Post

    ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...