We narrowed to 430 results for: {{{s}}}

1. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

2. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer...

3. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also...

4. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling...

5. Transferable Skills Guide: Career Planning Resources

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   Blog Post

   ...20140417174121-34888774-linkedin-etiquette-guide-20-do-s-don-ts Resources for getting into grad school: https...

6. Fluorescent Proteins 101: Fluorescent Protein Timers

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   .... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

7. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli...

8. MXS Chaining

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   Blog Post

   ...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with...

9. Plasmids 101: Gateway Cloning

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   Blog Post

   ...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers...

11. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

12. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform...

13. Plasmids 101: Codon usage bias

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    Blog Post

    ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...

14. How to Be an Excellent Trainee

    Type

    Blog Post

    ...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...

15. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

16. Getting to Know Expansion Microscopy

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    ...992. https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID...

18. DNA Purification Without a Kit

    Type

    Blog Post

    ... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ...

19. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome...

20. Bioinformatics at Addgene

    Type

    Blog Post

    ...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...