We narrowed to 435 results for: {{{s}}}
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TypeBlog Post...following four questions to figure out which method(s) work best for you, your audience, and your science...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post.... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...Nature Methods 9, 787–790 (2012). 3. Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli... -
MXS Chaining
TypeBlog Post...: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with... -
Plasmids 101: Gateway Cloning
TypeBlog Post...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry... -
Plasmids 101: Codon usage bias
TypeBlog Post...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded... -
AAVs in Retinal Gene Therapy
TypeBlog Post...dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little bit of luck A pioneer... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...traces in the region around the guide binding site(s). The sequence base calls from the .ab1 file are also... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific labeling... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform... -
Great Results Start with Great Standard Curves
TypeBlog Post... standard, standard dilutions, and unknown sample(s) in the same buffer if possible, or very similar buffers... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD... -
Getting to Know Expansion Microscopy
TypeBlog Post...992. https://doi.org/10.1038/nbt.3625 Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical... -
Bioinformatics at Addgene
TypeBlog Post...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...