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We narrowed to 963 results for: APP

Showing: 361 - 380 of 963 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...through an electric field to deflect them into appropriate containers. The machine is useful for distinguishing...on in vitro studies where it was difficult to apprehend the key role of specialized cell types and organs...Good two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins...this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely tune the...creation of mouse lines that can be used in fate mapping experiments. Immune response dynamics are much ...

  2. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post
    ... is supposed to be impartial. The best science happens when scientists have 1) the courage to make mistakes...degree. It was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying...tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how to engage with friends...galilei-dialogues-concerning-two-new-sciences). This is the approach policy makers take in abusing climate science ...defund science and stay silent. I was overwhelmingly happy to see so many others come out who feel the same...

  3. Science as Art

    Type
    Blog Post
    ... a serious academic subject, it is now being appreciated on an aesthetic level as well. Beautiful microscope...can be used as a way to better understand and appreciate the organism, for both scientist and non-scientist...the difference in some areas of conservation.” Appreciating science as an art form taps into an emotional...matter is seen to have increased value and is appreciated by a wider audience for more than just its biological...

  4. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...for both transient and stable gene expression applications in plants. This smaller, easily customizable... susceptible to Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...only for plant engineering and biotechnology applications, but also for foundational studies of promoter...

  5. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...defining a couple of key terms is a better approach. Also, appealing images and colors draw people in. Drawing...should be. He debated taking a position as an application support/sales representative in industry or a...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...

  6. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research... water repositories in Mexico. At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  7. Hot Plasmids - October 2022

    Type
    Blog Post
    ...a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein...in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved genetically-encoded...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

  8. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...Principal Scientist or Scientist I/II. Promotions don’t happen overnight though, and it might take longer to advance...

  9. An Addgene Summer Internship

    Type
    Blog Post
    ... had spent most of my junior year stressed out applying for summer programs. I spent one day at Addgene...internship opportunity taking place next summer.  I applied to Addgene and began the interview process with...that my decision deadline would be extended. I appreciated how flexible Amy was, which was the impression... before stepping foot into the lab itself. I appreciated that Addgene was treating all the high school...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...a variety of other types of vectors for most applications used to study gene function. From making transgenic...

  11. Advice for Choosing a Research Project

    Type
    Blog Post
    ...project that combines an exciting new method or approach with another in a way not done before in your ... - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko advises students... technology to identify a problem, asking what approach will I take and what can I learn, learning the...importance of choosing a solid research topic and approach. At Addgene, we've heard of labs where new hires...

  12. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into the CRISPR...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,... multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out...for your research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...revolution in biological research. We’re incredibly happy to see that one of our career posts has made it ..., we hope we can help all researchers achieve happiness in their careers regardless of their end goals...addgene.org. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101...some common controls and why you might need them. Happy Reading!   Resources on the Addgene Blog Download...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part...elements. At the time, the use of episome seemed appropriate, especially since the Fertility, or F-factor ...seen as an ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded...Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about the history of plasmids...

  15. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of...Institute focuses on proteomics: developing and applying new technologies to quantify proteins in tissues...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...used to label all proteins within a radius of approximately 20 nm of a specific DNA sequence in only 1 min...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in... same species as your primary/sample, and then applying this mixture to your tissue (Tuson, et al., 1990...After washing away unbound fragments, you can then apply your primary and secondary antibodies as normal....Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  17. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

  18. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...Quake Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...stability under harsh conditions. One example application from the publication is the removal of unwanted...

  19. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ... transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) Does your TTO ...periodically for possible new awardees, and we’re happy to share stats and strategies with tech transfer...executed between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene...executed between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA...

  20. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... typical genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of...since the majority of the viral genome has been swapped for alternative genetic cargo, they can’t reproduce...viral preps available for a variety of broadly applicable and popular viral vectors. Check out our viral...should you opt to make a virus instead of just zapping your cells? That really depends on two things. ...
Showing: 361 - 380 of 963 results