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We narrowed to 970 results for: Abo;

Showing: 361 - 380 of 970 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...plasmids, GFP knock-in cell lines can be created in about a month and a half, without the need for complicated...more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking a ...Directed Repair and Non-homologous End Joining Read About Other Genome Engineering Tools like Sleeping Beauty... Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages Find CRISPR Plasmids...

  2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt...tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We’ll start ...discovery and therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section...Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine...on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit...

  3. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Homology-Directed Repair Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA Editing with ...

  4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Robert Hurt and Mohamed Soufi. So you’ve learned about the Modular Cloning (MoClo) system and what it can...these would be interchangeable between different laboratories. Therefore, choosing acceptor vectors and parts...Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning videos made by the Nicola...system, are an invaluable resource for learning about MoClo in an in-depth and easy-to-understand guided... from Addgene will most likely arrive in your laboratory as plates of frozen glycerol stocks. It is very...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...a less tangible form: knowledge and information about the materials in our repository. We started to increase...by making it easier to find materials and learn about them. As early as 2007, we began grouping similar...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...updating and expanding our website, we’ve learned about the importance of smaller, systemic changes, like...

  6. Plasmids 101: Plasmid Incompatibility

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    Blog Post
    ...101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning Resources... plasmids within the same bacterial cell, think about whether plasmid incompatibility will affect your...this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics...of the microbiological world. (Want to know more about these plasmid types? Read our Plasmids 101: Environmental...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...brainstorming sessions.  Allow the group time to think about the problem before you meet. This holds the dual... meetings run smoother when people have thought about the issue ahead of time. Encourage dissent. The ... decisions while encouraging the group to think about alternative options. When a devil’s advocate is ...a few minutes every month dedicated to thinking about the future will improve your ability to innovate...to be able to continue our experiments. Thinking about these plans before you’ve run out of consumables...

  8. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...complete editing process. The MUCICAT system While the above systems are great for experiments that require only...serve to interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system...when compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine ...Multiple induction systems to choose from. Ready for metabolic engineering with improved cargo dedicated to gene...other CRISPR methods for bacteria Find all articles about microbiology Resources on Addgene.org: Visit the...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...deletions, the ability to analyze and interpret data about these types of edits is critical to the CRISPR workflow...Distribution and Traces - provides particular details about the indel profile of the edited sample. You can ...sequence base calls from the .ab1 file are also shown above each trace. The horizontal black underlined region...us know how it works for you! Read more details about the ICE tool in our bioRxiv paper. The new ICE CRISPR...guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi worlds or exploring nature in the...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...a single grant. Either way, think realistically about the time and resources required to feasibly accomplish...interrupted frequently by committee members. As mentioned above, plan to give your 10 minute overview and address... list of common questions, check out this eBook about applying for faculty positions. You need to practice...program. You should be proud of it and be excited about getting the opportunity to realize your vision. ...blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...

  11. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...gene, Xw, except for a single base. If you heard about CRISPR, one question probably comes to mind: can...compatibility All of the base editors mentioned above are SpCas9 nickase-based and depend on the presence... HDR methods for rice crop improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor,...The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional resources on the Addgene blog...Download the CRISPR 101 eBook Read our blog posts about plant biology Resources on Addgene.org Find all...

  12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...target metabolite creates an aptamer that will only bind to DFHBI in the presence of that metabolite (Fig...approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (or, fluorescing...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources...

  13. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...optimization within your experiment. Read on to learn more about the various controls that can be used for plasmid-based...: Expression Level of Gene X The results shown above are from a single experiment wherein Plasmid A (...be compared. The Empty Vector Control (mentioned above) could also serve as an important negative control...experimental cells. For example, in the experiment above, Plasmid A contains an shRNA that targets human ... times and on different days. Using our example above, we would test different preparations of Plasmid...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...time I heard about FRET during a journal club, my guitarist brain automatically thought about the raised...you would say. The student was of course talking about the now well-known FRET, aka Fluorescence (Förster...other. FRET reduced the scale of our perception about molecular interactions. What is FRET? In their ...Resonance Energy Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein...

  15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... this chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error...condition 2.” By eye, the loading control appears about the same between the two conditions. But it isn’.... However, it throws both assumptions discussed above into question. Assumption #1: The loading control...searching the literature to see what is already known about your housekeeping protein in your context. If you...step from the POI blot, you don’t need to worry about the linear detection ranges of multiple targets....

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ... the Addgene blog Read more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide Browse our entire COVID...

  18. Five Popular Model Organisms

    Type
    Blog Post
    ...the time between being born and giving birth - of about 10 weeks. Adult mice reproduce as often as every...are great model organism not only for the reasons above but because they actually share many biological ...cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes have a counterpart in humans ... become an important model organism. They share about 70% of their genes with humans and 85% of human ...in the popular model organisms series, and read about new systems being developed for emerging model organisms...

  19. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...Synthetic Biology One of the best ways to learn about a new field is simply to know where to look and ...might be helpful, please reach out to me by email.  About the author Niko McCarty is the editor of Asimov ...curriculum at MIT. He also writes Codon, a blog about biotechnology.    ...

  20. A Control for All Seasons

    Type
    Blog Post
    ...subjected to the experimental condition. In the example above, a negative control could be a third group of mice...in the experimental protocol. In the experiment above, you could repeat the tau western blot several times...Baseline secondary signaling levels In addition to the above-mentioned safeguards, you’ll need to test the secondary...Flow cytometry In addition to the controls listed above, viability and compensation controls are recommended...technique before starting. For more information about antibodies and antibody-based experiments, check...
Showing: 361 - 380 of 970 results