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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 712 results for: BASE

Showing: 361 - 380 of 712 results

  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent...

  2. Engaging with science and society at pgEd

    Type
    Blog Post
    ... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... modified) for optimal stability and efficacy.  Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...

  7. Lentiviral Prep Service

    Type
    Collection
    ...Lentivirus Browse our list of available viruses based on plasmids in our repository that express fluorescent...

  8. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...conclusions. Antibody engineering also benefits antibody-based therapeutics. Whether antibodies are used directly...measurement of antigen-antibody interactions in a plate-based format, making it suitable for comparing relative...biology, and protein engineering to develop antibody-based cancer therapeutics.    References and Resources...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... community. The Penn Vector Core produced viral-based vectors for distribution via Addgene. For a full...look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors ...still available, other serotypes may be available) Based on low demand, production of some viral vectors ...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,...

  12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    ...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data...

  15. dTAG - You're it!

    Type
    Blog Post
    ...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype...
Showing: 361 - 380 of 712 results