We narrowed to 571 results for: BLI

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   ...option is to perform a hierarchical assembly, assembling fragments in multiple steps to avoid using multiple...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...

3. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

4. Hot Plasmids - February 2022

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   Blog Post

   ...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ...

5. OCaMP: A New Calcium Indicator for Neural Imaging

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   Blog Post

   ...lasers, with two-photon excitation at 1030 nm, enabling two-photon imaging without compromising on brightness...

6. GCE4All: Making Genetic Code Expansion Accessible

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   Blog Post

   ...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...

7. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   ... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...

8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ... from this collection in your experiments and published your data, please fill out this form and we’ll...

9. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   ...expression under low temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic...

10. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ... gene. Mismatch nuclease assays are completely “blind” to this very important difference (Figure 3). ...

11. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post

    ...Wyss Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral...

12. Behind the scenes: Addgene’s new search engine and more

    Type

    Blog Post

    ... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    ...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...

15. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    Blog Post

    ...accounting from Babson College. He is a certified public accountant (CPA), certified information systems...

16. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly...

17. A Deep Dive into BioBuilder

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    Blog Post

    .... (and Mexico in 2018). BioBuilder textbook, published by O’Reilly Media. BioBuilder's open-access curriculum...

18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

19. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...

20. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...