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We narrowed to 644 results for: CAR

Showing: 361 - 380 of 644 results

  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

  7. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...

  8. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to the light, where they appear...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1: Left: recombined...and adopts an open conformation in the absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...can be transfected with your transfer plasmid, carrying your gene of interest, and the two helper plasmids...undergoing titering, to remove residual plasmid DNA carried over from the production process. For more details...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...expect to see for the unknowns in your experiment. Carefully set up a standard curve dilution series that takes...increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline that's ...

  17. Plasmids 101: Protein tags

    Type
    Blog Post
    ...translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not...handbooks for protein purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts ...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing contributed to the update...PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics....
Showing: 361 - 380 of 644 results