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We narrowed to 933 results for: COMP

Showing: 361 - 380 of 933 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ... assortment toxins to claim territory or kill competing microorganisms, but did you know bacteria also...subunit of DNA gyrase and trapping it in the cleaved complex. This results in DNA breakage and cell death. Conversely...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...populations to retain more of these plasmids as compared to those without the restriction modification ...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...K. Burley. "Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition....

  2. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...proteins. lon protease: Strains where this is completely deleted (designated lon or Δlon) similary reduce... and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys comes in two ...measures that can help get you back on track: Compatibility: Double-check your plasmid backbone and expression...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an...strain for example, nor will a p15 plasmid be compatible with a pLys strain. Your strain may require additional...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post
    ...across the entire blot. So how can you accurately compare different lanes? In relative quantification, the... conclusions, and it’s also problematic for computational quantification. Outside the linear detection...individual protein. This ensures you are truly comparing the abundance of your POI as a fraction of the...detection ranges of multiple targets. Convenient! Comparison of loading control approaches Cartoons are nice...all, but we’ll leave you with this real-world comparison of different loading control approaches from ...different methods of quantifying total protein are compared. While a higher slope indicates higher sensitivity...

  4. Plasmids 101: Biotinylation

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    Blog Post
    ...metabolites. Biotin acts as a carboxyl carrier from one compound to the other. These biotin dependent enzymes are...discovered in the 1960s, strepavidin. The Biotin-avidin complex can then be used and/or manipulated for a scientist...immunoprecipitation experiments. Biotinylated proteins or complexes can be easily purified using columns or beads ... sample. Any free biotin-binding sites on this complex can bind to your biotinylated antibody. This creates...creates a large meshwork of biotin-avidin complexes bound to your protein of interest leading to amplification...This strategy is referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information...

  5. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post
    ... different universities are trying to compile more comprehensive demographic information about the postdoc...Postdoc: An Institutional Perspective. This lack of complete information does not, however, prevent us from...from the career panel was that there are many companies and organizations (including Addgene), that need...scientifically minded, and able to communicate complex information - all traits you are likely to find...others within their labs - as a sort of over-compensation, these mentors can treat the underrepresented...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...transposition. INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins...transcription by physically blocking the elongation complex from binding the DNA or extending the transcript...transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a transcription...transcription factor called SoxS for CRISPRa. Adding complexity, they found that they could also include a CRISPRi...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one... targeting approach is then used to direct the complex to a specific locus. This approach provides a more...

  7. Podcast: Interview with Addgene's Founders

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    Blog Post
    ... received his PhD in Computer Science from MIT. His research focused on computer systems and networks.... 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing...Kenneth received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master...and a Masters in Engineering from MIT, both in Computer Science.   Special thanks to our sound engineer...

  8. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

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    Blog Post
    ...Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however...Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics to developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To...using Sample 6 and why? 24:41 - 29:00 : Dr. Koeris compares and contrasts being a graduate student with being...personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is it to ...

  9. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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    Blog Post
    ...reporters Difficult to Multiplex: FRET reporters are comprised of two fluorescent proteins with very specific...encoded fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase...for kinase activity.  Advantages of KTRs When compared to FRET reporters for measuring kinase activity...of KTRs KTRs also have some disadvantages when compared to FRET reporters. First, KTRs monitor global ...measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus...

  10. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...possibilities in the engineering of complex gene networks. A comparable system for targeting specific sequences... of RNA is highly desirable for extending the complexity of genetic circuits, allowing for tighter spatio-temporal... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA..., designer PUF proteins can be fused to other components to lend them many other functions for use in ...

  11. Tag Your Favorite Yeast Genes with Ease

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    Blog Post
    ...markers. These are especially useful in protein complex studies where tagging of multiple protein products...for easy capture and detection of proteins and complexes, without the artifacts sometimes associated with...Formosa, at the University of Utah, has built a complete collection of yeast tagging modules with each ...ideal for tandem affinity purification of protein complexes. John Pringle and Jürg Bähler have deposited a...GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with...

  12. Working 9-5: an Addgene Co-op

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    Blog Post
    ...The application process When I first saw the comprehensive job description for this co-op, I was immediately...description of the tasks I would be responsible for, in comparison to the other positions listed with a much less...training, I was gradually introduced to Addgene as a company and given more details on what my position entailed...they do and how it relates to other teams at the company. This provides new (and old) employees with information...Northwestern University. She is one of several students completing their co-op at Addgene. This is her second contribution...

  13. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post
    ...without the exo/polymerase generation of annealed complementary regions. Why use a high fidelity ligase? Numerous...the Ligase Detection Reaction (LDR), probes complementary to the sequence of interest are annealed to ...separate pools were prepared, each with a different complement strand covering all 16 possible NN pairs in the... system for direct identification of allelic composition of mutated DNA in a mixed population of excess...JS, et al. "A high-throughput assay for the comprehensive profiling of DNA ligase fidelity." Nucleic acids.... Pascal, John M., et al. "Human DNA ligase I completely encircles and partially unwinds nicked DNA." ...

  14. Build Your CRISPR Vocabulary

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    Blog Post
    ...Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an...proteins, and a CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer..., and the spacer sequence is used to target complementary DNA sequences. In laboratory settings, an sgRNA...whose sequence is not checked. Assuming full complementarity, its sequence matches the guide sequence. PAM...either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide and target...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post
    ...give you the reverse sequence, complement sequence, or reverse and complement sequence.  Filter DNA tool:...well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in... tools help with that.  DNA sequence reverse complement tool: Enter a DNA sequence and the program will...DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation reaction...

  16. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...response to a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular... Erp, et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen... known as opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM...pathogen activates the immune system's classical complement pathway, starting a cascade of events that results.... PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate and adaptive immune responses...

  17. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type V-K CAST systems...CAST activity allowed certain mutated phages to outcompete their brethren, and the researchers isolated ...evolved and engineered adjustments to the other components. It drove 19% integration in HEK293T cells (more...that kind of gene treatment. The researchers also compared evoCAST to the Liu lab’s own PASSIGE (Prime-Editing-Assisted...

  18. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post
    ...Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital...from.” Working at eScience Labs, an educational company that creates hands-on science lab kits and digital... editing.” It was for Cactus Communications, a company that provides scientific editing services. “I was... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...what it’s like to work for a science education company. And make sure to subscribe to the Career Channel...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ...Visualize components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...process or detect a given molecule. The sensor component is usually a protein that undergoes a conformational...biosensors can be targeted to a particular cellular compartment such as the nucleus, mitochondria, endoplasmic... biosensor: which fluorescent protein is most compatible with your lab and experimental setup? Consider...

  20. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse...Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just...heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively remove a stop...microscopy, they also show that this method is compatible with dead cell staining using propidium iodine...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...
Showing: 361 - 380 of 933 results