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We narrowed to 824 results for: EED

Showing: 361 - 380 of 824 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...full-time and abandons all safety nets.” Raising seed money. “Before seeking funding, ask yourself if ...solid, and “the pitch is packaged to perfection”, seed money will hopefully be raised and the company may...Work fast. Be flexible. Be quick at failing and succeeding. Take home Starting a company requires dedication...

  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...selection markers in yeast and provides the element needed to overcome the auxotrophy as well as additional...means of selection: A specific selection marker needs to be used with a yeast strain deficient in that...element pairs must be utilized or a new combination needs to be created in advance of the experiment. The ...

  3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...channelrhodopsins in one region of the brain, which is needed since current channelrhodopsins tools have low ... tissue (~1 mm3). Current channelrhodopsins also need high-intensity light for activation which requires...transduction of the nervous system which eliminates the need for intracranial injections. When packaged in PHP.eB...

  4. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...kb, meaning that your gene of interest (or genes) need to be 4.7 kb or less to fit in a single rAAV. For...gene of interest is truly small (<2.5Kb) and you need expression in a hurry, self-complementary AAV (scAAV...dsDNA in the nucleus and is a good option if you need rapid transgene expression, since you don’t have...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    ...your experiment? What about all the reagents you need to order? Sometimes, it feels like an eternity of... order or try to make it in your lab (but if you need a lot of plasmids, it’s almost always faster to ...right one can save you time if you’re working at speed or money if budget is a concern. The faster methods...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... its normal neuronal context. In other words, I needed a method for visualizing protein turnover in situ...incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences 51.4 (1964...puromycin of amino acid incorporation into protein." Proceedings of the National Academy of Sciences 45.12 (1959...

  7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CombiGEM-CRISPR experiments The first thing you will need is to get the list of effective gRNA sequences targeting... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016...

  8. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... such as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and... Exposure time is the amount of time the sample needs to be under light to discern the signal. If the ... 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics...

  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...mechanisms and, eventually, develop treatments, we need tools to specifically edit methylation sites.  Step... DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise it will continue to ...nuclear environment. One good system to balance these needs is the lentiviral-based expression of floxed dCas9...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting...chemogenetic system To set up a chemogenetic system, you’ll need to choose a method for targeting receptor expression...cell types, or even subcellular regions of a neuron need to be targeted. Targeting is also important in the...

  12. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...number, media volume, and plasmid concentrations will need to be optimized prior to starting. We recommend ... experimental volumes accordingly. While you may need to make some final adjustments, this is generally... to start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt...

  13. Tips for Technical Support Calls

    Type
    Blog Post
    ... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...know (nicely, please!). We can’t improve without feedback. And if you had a very positive experience, also...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...switch can be created for your specifc experimental needs. How does the FLEx switch work? Let’s say you want... switch to successfully work, the cassette would need to contain the BFP coding sequence in the sense ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

  15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...marker proteins, however, in this case the cell needs to be fixed and the cell membrane permeabilized ...problems associated with fluorescent imaging such as bleed-through (the incorrect detection of one fluorescent...baseline rule, the emission spectra of the selected FPs need to be sufficiently separated, most commonly FPs ...

  16. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...signal input to readable output, which generally exceeds that of the VSD GEVIs. However, since rhodopsin... specific targeting of these voltage indicators needs to be cell type specific as well as specifically...10.1186/s12915-019-0682-0. Beck, C., Gong, Y. A high-speed, bright, red fluorescent voltage sensor to detect...

  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...note, there is no minimum number of markers that needs to be used with spectral flow—you can run a three...co-expression among cellular markers You’ll also need to know if markers are co-expressed in your cells...have a level of background autofluorescence that needs to be accounted for in order to distinguish the ...

  18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates and sequencing... such a scale, automation is crucial, both for speeding things up and for standardizing the process. When...mutates HDR donor templates to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...planning, you’ll know exactly what materials you’ll need to bring into the hood and can wipe them all down...cellular metabolism that is toxic to the cells), and need to be split very soon. There is power in numbers...or contaminated. This also ensures that anyone needing new cells in the lab can take a vial of the ‘mint...
Showing: 361 - 380 of 824 results