We narrowed to 447 results for: ENA;

1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

   Type

   Blog Post

   ...biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing....

2. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,...

3. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you...

4. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by cutting the vector with...

5. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing...

6. MXS Chaining

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   Blog Post

   ...directed localization of the fluorophore, and thus enabled labeling of particular structures (Table 1). Each...

7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet to be fully realized...

8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   Blog Post

   ...chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base...

9. Summer SciComm Series: Modes of Communication

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   Blog Post

   ...come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms...

10. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

11. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...substitute for sound techniques, routine machine maintenance, and critical interpretation of the results.  ...

12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ...of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists...

13. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

15. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don...

16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451...

17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

18. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ...FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control. What is FLP...

20. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ... variety of plasmids. Speed The Gateway system enables the generation of the expression construct in only...