We narrowed to 438 results for: ENA;

1. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet to be fully realized...

3. Summer SciComm Series: Modes of Communication

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   Blog Post

   ...come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms...

4. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing...

5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   ...of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists...

6. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

8. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ...home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don...

9. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ...classroom, in their lab, in their experiments, to enable virtual learning during the pandemic. Feedback ...

10. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...using/depositing. Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene...

11. Hot Plasmids Spring 2024

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    Blog Post

    ...PE7, it will be exciting to see future studies enabled by this tool as well as additional potential improvements...

12. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp...

13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, ...

14. Plasmids 101: Gateway Cloning

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    Blog Post

    ... variety of plasmids. Speed The Gateway system enables the generation of the expression construct in only...

15. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...moderators for keeping the quality high and thus enabling a productive discourse. They even advised me on...

16. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...

17. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous...

18. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...gene system to clone multiple protospacers which enables us to target more than one genomic site simultaneously...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...restriction-modification systems mediate plasmid maintenance." Journal of bacteriology 177.12 (1995): 3451...

20. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...