Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 711 results for: ESC

Showing: 361 - 380 of 711 results

  3. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...targeted approach using RNPs has recently been described in vitro, with the hope of eventually using this...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...revealed that cell lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms...are more common in individuals of African descent.Correcting the bias  While their thorough analysis uncovered...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually have shorter mRNA and ...tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and... unnatural head groups for applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD...

  15. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class of...

  17. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...term, but are worried that this might sound condescending, consider explaining the term on a slide but...

  18. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control of neural...

  19. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...specific substrate. The emitted light, or chemiluminescence, is detected on x-ray film or with specific..., Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019) Antibody validation for Western blot...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...
Showing: 361 - 380 of 711 results