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  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was coined...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally,...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...the results are correct. Primers and probes can interfere with amplification reactions, and reagents can...

  7. Lentiviral Guide

    Type
    Guide
    ...respect to the precise nature of experiments being performed, and your biosafety office can provide more information...

  8. Technical Design of a Western Blot

    Type
    Blog Post
    ...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...interest is not available in a pure form, you can perform relative quantification, which measures protein...

  10. Molecular Biology Reference

    Type
    Guide
    ...Plasmids 101: Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting...

  12. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

  13. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...

  18. Deck the Lab 2022!

    Type
    Blog Post
    ...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...
Showing: 361 - 380 of 631 results