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Showing: 361 - 380 of 1076 results

  1. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...science on. Practice self-care and stay connected Find time for exercise. Many studios and gyms are now... to support your favorite local exercise spot or find something new. We usually have yoga class twice ...great activity for both exercise and clearing your mind. And because the outdoors is so huge, these are ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...Work on personal development Take the myIDP (individual development plan) from Science to identify skills...and set up activities that kids can do more independently. For example, they could do something like puzzling... kids. Plan out meals and snacks ahead of time.  Find learning activities for kids. For example, Skype...

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...at Addgene.org Find Fluorescent Protein Biosensors Browse All Fluorescent Proteins Find AAV Tools References...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations...anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...pRSET: Bacterial expression vector, use with IPTG induction to express soluble protein CAG: Generic strong... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  3. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding protein so ...are able to quickly sequence an individual’s genome. But when they find genetic variants, what does that...Get the basics on GFP Find microbiology blog posts Resources on Addgene.org Find fluorescent protein ...” Unger began with the a bacterial periplasmic binding protein, one similar to the protein which gave ...bioreporter such that fluorescent proteins expression is induced prior to inoculation with IPTG. In the absence ...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post
    ...Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday...award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins (FLiPs) Fluorescent proteins can be applied...these photoswitchable enzymes Fluorescent Light-inducible Proteins, or FLiPs.  Xin later optimized these...spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She hopes that other researchers...create a wide variety of enzymes and proteins with inducible activity and recently presented her work at the...sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima specifically proposes...with bacteria engineered to produce HMOs, sensor binding results in an increase in fluorescence of cells...

  5. 5 Great Apps for Lab Life

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    Blog Post
    ...buffers to use and that indicates if a sequential digest is needed. The enzyme finder is also a very helpful...learning proper cell culture technique, I tried to find an app that could help me with calculations. This...to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest. Lastly...with research! It is always important to know what kind of reagents you work with in experiments. Hence,...app has a search function that allows the user to find an MSDS for a lab chemical of choice. Now, I can...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource to..., J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split Calculator by ...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...selection strategy is not enough on its own to help you find your desired plasmid. In many cases, you will need...survive and you will need to sort through them to find the desired clone. Do your selection, then screen...𝛽-galactosidase, it turns into an insoluble blue pigment. Find plasmids for blue-white screening at Addgene! Some...proper positive and negative controls for your PCR.  Find the details and learn more about colony PCR in this...into your insert. Check out our Vector Database to find plasmid maps of the more commonly used backbones...you can use to confirm your clones. You can also find a list of popular sequencing primers in our Molecular...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...cannot offer. Furthermore, companies across most industries tend to hire their former interns to fill entry-level... in.     STEAMid is an app that helps you find internships and scholarships in different subject...to submission. When in doubt, consult the web to find the tools that can help you craft a solid personal...Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle to vouch for the ... superb and detailed portrayal of yourself. Be mindful that January and February represent peak season...for internship applications. Thus these noble individuals are inundated with requests. The easier you make...        5. Application submission  Set a calendar reminder for every deadline and shoot to submit your application...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...functions. Photoswitches are engineered molecules that induce conformational changes with light stimulation to...activation have been developed to selectively study individual types of GPCRs with greater spatial and temporal...of the receptor, similar to what the endogenous binding partner would do. This allows precise control over...molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor that change shape...concept, except the ligand is attached to a protein binding partner or nanobody that targets the native receptor... when exposed to light, the ligand engages the binding pocket and activates the receptor. Created with...absence of light the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, so be...different ways to ultimately achieve success, we find it is usually faster and easier to do the validation...it can be ubiquitous, cell-type selective, or inducible.  Ubiquitous promoters  These often-strong promoters...over time, resulting in decreased expression (Damdindorj et al., 2014; Nieuwenhuis et al., 2021). It is... Attribution 4.0 International License.   Inducible promoters  This type of promoter allows you to... turn off, gene expression by administering an inducing agent like doxycycline (Favre et al., 2002). They...experimental output. You’ll also want to keep in mind the serotype you’ve picked, since that can affect...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...controls.) 27.6% of embryos carried indels at the mutation site, indicating that repair proceeded through NHEJ...move forward from this work. Experiments & major findings Let’s start with the paper itself: Ma et al. were...electroporated them with an ssODN repair template in induced pluripotent stem cells made from the patient. With...allowing repair to take place before DNA replication. Indeed, they observed that 41/42 embryos were non-mosaic...using the provided ssODN template. In fact, this finding led the authors to title the paper “Correction ...pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing...pregnancy, the researchers were able to examine each individual blastomere to determine its genotype. However...

  11. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...on the Addgene Website Find CRISPR Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources...combined with the weak separation afforded by gRNA binding at non-target sites would theoretically decrease... Figure 1: Strand separation and target DNA binding to the Cas9 nuclease. Stable strand separation ...similarly thought that if they could weaken sequence independent interactions between Cas9 and DNA, then they ...SpCas9-HF1 each stall in an inactive form after binding to a target site and propose that this stalling...Science (2015): aad5227. PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu...effects." Nature (2016).  PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene Find the ClonTracer...exciting new tool that allows researchers to individually label millions of mammalian cells through lentiviral...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...these groups with the same drug regimen, they would find one of two outcomes (see schematic depiction below...ClonTracer library allows a researcher to label individual members of a starting population of cells with...multiplicity of infection (MOI) such that each individual cell receives only one barcode. The abundance...is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to ...

  13. Plasmids 101: Cre-lox

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    Blog Post
    ...: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual switch regions...PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...-lox system is a technology that can be used to induce site-specific recombination events. The system ...key advantage of the system is that Cre acts independently of any other accessory proteins or co-factors...recognition sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric ...certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre...unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence...

  14. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same three nucleotides...Fidelity, it turns out, might depend more on the individual sequence(s) than on how well that sequence fits...Pryor et al., 2020), and randomly selected non-palindromic pairs allows for high-fidelity assembly for up...potential for mismatches, so you can redesign individual pieces as needed to improve fidelity. NEBridge...tool for you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...define the number of fragments, and the “search windows” you’d like the tool to use to identify fusion ... return the highest-fidelity overhang set it can find for those parameters. For even more specificity,...

  15. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...historical ties to psychology and philosophy (the mind-body problem). Because of the availability of tools...between animals as olfactory stimuli that were left behind from a preceding animal (ex: urine) can be distracting...Animal Strain, age, sex, housing conditions (individually versus in groups), health status, diet, cage...differences in behavior observation); experimenter blinding to avoid bias Genetic manipulation in behavioral... neuronal targeting after stereotaxic injection. Find more details about choosing AAV or lentiviral vectors...about two weeks before the behavior test. This time window between injection and testing will vary depending...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... International C. elegans meeting, I was again reminded of the extent that C. elegans researchers embrace...worm community where one can publish brief novel findings. These results are normally one figure and can...This a great resource for scientists to publish findings that don't fit into larger publications and would...embryo when all essential embryonic genes are individually depleted via RNA interference (RNAi). Scientists...tools for the community. Plasmids for the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015...controls the expression of a GAL-4 driver that can bind to activation sequences upstream of genes to stimulate...expanded on this tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are...

  17. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in ...two general categories: rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase...common bacterial expression plasmids use Rho-independent terminators, which include naturally occurring... high-efficiency terminators such as T0. Rho-independent termination is also known as intrinsic termination...secondary structure and sequence conserved Rho-independent termination annotation for 90 bacterial elements...CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=39322032   No terminator is 100% efficient... than Polymerases I and II, which both require binding of termination factors. Although both are termination...

  18. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... all”. Manage the individuals in your team: learn what motivates them (keep in mind that it can be very...it will take time so be patient and keep an open mind. First let’s clear up a misconception about being...have reports with different levels of experience), find out what their learning styles are and capitalize...benefit of the team. By properly managing the individuals in your team you will have a much better chance...(needs to) feel valued. Talk to your manager and find a mentor Your manager should support you, just like... how to face a particular situation. But keep in mind that a good manager should help you work through...you and you need to encourage them to speak their minds. They will make mistakes and you will take responsibility...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...in the genomic locus. The first two breaks are induced via a generic PITCh-gRNA; the third break by an...flanked by 5' and 3' microhomology and PITCh-gRNA binding sites. This PITCh plasmic is cotransfected with...optimized the PITCh-gRNA for minimal off-target binding in mammalian genomes as assessed by CRISPR design... and 3’ junctions, respectively. These results indicate that PITCh is a robust method for genomic insertion...staggered pattern, it is thought to be ideal for HDR-independent knock-ins, but this possibility is still being...systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids from this publication at Addgene. ... Cells.” Cell Rep. 13: 621-633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ... of binding DNA to silica columns, it’s bound to glass syringe filters. Normally DNA does not bind silica...silica are commonly used to bind and purify DNA, cellulose also efficiently binds, or at least entraps nucleic... can be stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass... and 2) it helps DNA stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation...mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids, so placing... Addgene blog Read our Plasmids 101 blog series Find other plasmid protocols and tips Browse our plasmid...plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning Read...
Showing: 361 - 380 of 1076 results