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We narrowed to 1,034 results for: LAS

Showing: 361 - 380 of 1034 results

  1. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...AAV vectors is the Allen Mouse Brain Connectivity Atlas, a project to map neuronal connections in the mouse... be found on the Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...

  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...taken up the plasmid. This can serve as an alternative to antibiotic selection. Plasmids of this type .... Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed...each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split... marking/selection: Expression constructs like plasmids often include GFP as a marker to help identify...techniques in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide... plasmids 101...

  4. Countdown to Halloween @Addgene

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    Blog Post
    ...love of plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our ...obviously make for good costumes), science and our plasmid collection (what science has been hot this year... ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, ...Office Team was back up to their creative hijinks last year. They showed us their dedication to great customer...

  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post
    ... of the Plasmodium falciparum genome. To put this last point into perspective, the Plasmodium falciparum... over 90% in their work editing the genome of Plasmodium falciparum (the parasite that causes malaria)...with the post-design process such as primer and plasmid design. Examples:  “multiplex design”, “multi-method... same organism could be used as a proxy for this last approach. Top tool for first timers: You need to...also the only tool I have come across to offer classes of parameters based on the type of CRISPR experiment..."Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature...

  6. What's New in CRISPR - May 2019

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE...paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool you’ve recently...

  7. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria... library or an especially low concentration of plasmid), you will benefit from purchasing competent cells...survivalTM, and Stbl3TM. How to transform your plasmid DNA If you decided to use commercial competent ...negative control (plate your cells without adding plasmid) to make sure you don’t have contamination.  ... Also conduct a positive control (test a plasmid that transforms well in other competent cells) to ensure...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge... to form. When expressed in the non-oxidizing cytoplasm of bacteria, scFvs accumulate in insoluble inclusion...expression in bacteria:  targeting to the oxidizing periplasm of bacteria to allow for proper disulfide bond...are redox mutants so they have a more oxidizing cytoplasm. expression in the presence of molecular chaperones...chaperones that help scFvs fold properly in the cytoplasm (Sandomenico et al., 2020). conversion to a cysteine...cysteine free format that’s easier to express in the cytoplasm of E. coli, although this is not possible for ...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  9. What's New in CRISPR - December 2019

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...RESCUE. Read the paper in Science Find RESCUE plasmids If you have a new CRISPR tool you’ve recently...

  10. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...Menassa's labs made the accidental discovery that laser treatment can photoconvert GFP from green to RED...Photoconverted GFP spreads through the cytosol via cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...

  11. Tag Your Favorite Yeast Genes with Ease

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    Blog Post
    ...Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place of the epitope...Jürg Bähler have deposited a large collection of plasmids for genome modification in yeast developed by ...Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome modifications in ...Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional ...

  12. Addgene is Expanding Our Viral Vector Service!

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    Blog Post
    ...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

  13. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...to once again reflect on our achievements in the last year and share the exciting things to come in the...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

  14. SunTag and Fluorescent Imaging

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    Blog Post
    ...scFV antibody, which is expressed from a separate plasmid. This system amplifies the intensity of the fluorescent...SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter...The high SunTag signal allowed them to cut their laser power by over 80% and still obtain a higher signal-to-noise...imaging deep inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked single molecules...basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it’s clear that the...Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  15. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

  16. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...

  17. Choosing Your Perfect Empty Backbone

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    Blog Post
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  18. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...articles (3/12/2014). With so many options for CRISPR plasmid tools and numerous experimental design decisions...genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR genome editing experiment...with expression, though it is possible for the plasmid to still express without the G. Because the G is...marker to select transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459...related to the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools...by a variety of labs and optimized for specific plasmids Links to different software to help you identify...

  19. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype...dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses...serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8  pAAV-hSyn-mCherry...channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA...55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-...

  20. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ...Greek for “self-eating”) is a process by which cytoplasmic material, including organelles, are targeted ... untargeted Rosella remains in the neutral pH cytoplasm where it emits red and green fluorescence. Following...clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused with a mitochondria-targeting...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe... autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana... Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent protein guide: Biosensors...
Showing: 361 - 380 of 1034 results