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Plasmids 101: Using Transposons in the Lab

Type

Blog Post

...first discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

...the haystack” type of problem and led to me to explore alternate approaches. Being a “lazy” geneticist...
Important Considerations When Using AAVs

Type

Blog Post

...171-182. PubMed PMID: 17328683. 11. Wouterlood, Floris G., et al. "A fourth generation of neuroanatomical...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

...for Designing Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing...
Deep Dive: Statistical Tests (Comparisons)

Type

Blog Post

...SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical facets of reproducibility...
Plasmids 101: Modular Cloning Applications and Kits

Type

Blog Post

...https://doi.org/10.1021/acssynbio.3c00167. Hahn, Florian, et al. “A Modular Cloning Toolkit for Genome Editing...
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Type

Blog Post

... by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins without...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

... Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical aspects...
Selecting Your Plasmid Purification Kit

Type

Blog Post

...kits are quick, easy, and don’t involve phenol-chloroform. The default option is to use the kit that ...
CRISPR 101: Cytosine Transversion Editors

Type

Blog Post

...apyrimidinic sites and abbreviated as AP sites. To explore this further, the team started engineering a CBE...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

...below. Figure 2: gRNA target sequences (colored rectangles) are cloned into various plasmids using...
Troubleshooting and Optimizing a Western Blot

Type

Blog Post

...need such precise measurements, you can use a colorimetric ladder, visible to the naked eye; a fluorescent...
Viral Vectors 101: Systemic Capsids

Type

Blog Post

...intravenous delivery by directed evolution of underexplored sites on the AAV capsid. Molecular Therapy -...
Adeno-associated virus (AAV) Guide

Type

Guide

...short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes enables them to...
Sequencing Primers

Type

Guide

...primer CAT-R GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer CMV Forward...

Showing: 361 - 375 of 375 results

Results per page:

Plasmids 101: Using Transposons in the Lab

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Important Considerations When Using AAVs

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Deep Dive: Statistical Tests (Comparisons)

Plasmids 101: Modular Cloning Applications and Kits

Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

Special Delivery: Fluorophore Targeting for FRET Studies

Selecting Your Plasmid Purification Kit

CRISPR 101: Cytosine Transversion Editors

CRISPR 101: Multiplex Expression of gRNAs

Troubleshooting and Optimizing a Western Blot

Viral Vectors 101: Systemic Capsids

Adeno-associated virus (AAV) Guide

Sequencing Primers

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Deposit Plasmids

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