We narrowed to 525 results for: MEA

1. Viral Vectors 101: The AAV Retrograde Serotype

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   Blog Post

   ...require receptor-mediated entry into the cell. This means that not all neurons and pathways will be transduced...

2. Antibodies 101: ChIP

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   Blog Post

   ...your output assay. A longer sonication time could mean needing to increase crosslinking to counteract the...

3. Antibodies 101: Isotypes

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   Blog Post

   ...expressing IgM. IgM has a pentamer structure, which means each IgM molecule has a binding capacity or valency...

4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...these plasmids available for scientists speedily meant that we had to revamp some of our quality control...

5. Behind the scenes: Addgene’s new search engine and more

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   Blog Post

   ...devil is in the details of course: what does “best” mean? and what “makes sense” to a user? Moreover, there...

6. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...direct comparison of different enzymes. CasPEDIA is meant to be a constantly updating database which serves...

7. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ...replication. ITRs are cis-acting elements, which means that they are in the same DNA molecule as the gene...

8. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ... from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope expressing...

9. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...memes was how I used social media as a scientist. I mean, I had a LinkedIn page and a Twitter handle, but...

10. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ...using AAVs because they provide a highly versatile means of delivering genes to many different cell types...

11. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ...be affected by methylation. This table is by no means exhaustive, so you may want to consult the REBASE...

12. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ... or other cells. It was made artificially. That means you aren’t getting any background or cross-reacting...

13. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...faster than HDR. NHEJ is consequently the principal means by which CRISPR/Cas9-introduced breaks are repaired...

14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...takes around 1 – 2 years. The product is the measure of success. Technology is proven in (A) Productize...

15. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    ... the discussion and for being “coachable”. That means the mentee needs to be open to change and constructive...

16. Protocol - Bacterial Transformation

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    Protocol

    ...bacteria but also because bacteria are used as the means for both storing and replicating plasmids. Because...applied to the cell/DNA mixture to induce membrane permeability. To do this you will need to have access to ...

17. Protocol - How to Ligate Plasmid DNA

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    Protocol

    ...each other. When the sticky ends are compatible, meaning that the overhanging base pairs on the vector and...average size genes and vectors, this ratio is really meant to refer to the molarity of DNA ends available for...

18. Antibodies 101: Conjugation

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    Blog Post

    ...2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https...

19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...are initiated before the cell has divided. This means that genes located near the origin will be over-...

20. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    ...with an anti-HA antibody and serves as a backup means of detection that’s independent of the reporter....