We narrowed to 391 results for: NAM

1. 10 Steps to a Perfect Science Talk

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   Blog Post

   ...accomplish all of these things naturally than to give a dynamite presentation?  To that end, let’s chat about giving...

2. Evolution of Lab Techniques

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   Blog Post

   ...and quantitative real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be ...

3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several store-bought...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   ...160 distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful technology...

5. Chemogenetics Guide

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   Guide

   ...Effector Molecules (PSEMs). PSAM domains were first named for the mutations that allowed them to respond to...Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020). Deschloroclozapine, a potent and selective...

6. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   ...(e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N terminal); 6xHis...

7. How to Design Your gRNA for CRISPR Genome Editing

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   ...the accuracy of a predictive score, as well as dynamic aspects of the target (e.g. accessibility due to...

8. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   ...Deisseroth, K. (2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202...

9. Is this the right place for me? 8 tactics for choosing a lab

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   ...destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation, sexual harassment...

10. Finding Your Perfect Job After University

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    Blog Post

    ...fairly unique situation, because it is a small and dynamic company and so it has the flexibility of a ‘start-up...

11. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...1415-1422. PubMed PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through ...

12. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    ...produced from long dsRNA via an RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded...

13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ... inverted in relation to the promoter, hence the name, double-floxed inverted open-reading frame (DIO)...

14. Plasmids 101: Using Transposons in the Lab

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    ...Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise, piggyBac was discovered in the...

15. Quick Guide to All Things Lentivirus

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    ... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...genetics.118.301775 Ghanta KS, Doksin GA, Mir A, Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer ...

17. Antibodies 101: Flow Cytometry

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    Blog Post

    ...to line up in single file in a process termed hydrodynamic focusing. This allows each cell to pass through...

18. Antibodies 101: Designing Your First Flow Panel

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    Blog Post

    ...fluorophores carry their emission peaks within their names, for example, BV421 and RB545. Fluorophores can ...

19. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post

    ..., USA 39.7392°N 104.9903°W - Erin Golden Hi, my name is Erin and I have spent the last 15 years training...

20. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different...