We narrowed to 403 results for: NAM

1. Important Considerations in Optogenetics Behavioral Experiments

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   ...methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell...

2. Plasmids 101: The Promoter Region – Let's Go!

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   ... the human ubiquitin C gene Constitutive  As the name implies, this promoter is ubiquitous. human beta...

3. The Advances Behind The World's First Personalized CRISPR Treatment

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   ...administered to KJ was designated NGC-ABE8e-V106W. The name is hardly flashy, but that mouthful reflects the...

4. Plasmids 101: Expressing shRNAs for RNA Interference

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   ...you might say. RNA interference (RNAi) is, as the name might suggest, an RNA-mediated gene silencing mechanism...

5. Antibodies 101: Beyond Surface Labeling

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   ...think of mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow ...

6. March for Science

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   ...science in government: 314 Action: This nonprofit named for the mathematical constant “pi” supports scientists...

7. Five Popular Model Organisms

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   ...cell signaling, aging, and mechanotransduction to name a few. For example, Andrew Leifer’s lab utilized...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...efficiency of the original APEX (Lam et al. 2014). They named this mutant APEX2.  Find the APEX2 plasmids! TurboID...

9. 10 Steps to a Perfect Science Talk

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   ...accomplish all of these things naturally than to give a dynamite presentation?  To that end, let’s chat about giving...

10. Evolution of Lab Techniques

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    ...and quantitative real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be ...

11. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several store-bought...

12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...160 distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful technology...

13. Viral Vectors 101: Optogenetic Tools

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    ...Deisseroth, K. (2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202...

14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...(e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N terminal); 6xHis...

15. How to Design Your gRNA for CRISPR Genome Editing

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    ...the accuracy of a predictive score, as well as dynamic aspects of the target (e.g. accessibility due to...

16. Is this the right place for me? 8 tactics for choosing a lab

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    ...destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation, sexual harassment...

17. Finding Your Perfect Job After University

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    ...fairly unique situation, because it is a small and dynamic company and so it has the flexibility of a ‘start-up...

18. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...1415-1422. PubMed PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through ...

19. Quick Guide to All Things Lentivirus

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    ... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ... inverted in relation to the promoter, hence the name, double-floxed inverted open-reading frame (DIO)...