We narrowed to 663 results for: PPL;
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TypeBlog Post...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ...
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How to Prepare for an Industry Interview
TypeBlog Post...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...the co-op application process, my advisor encouraged me to keep my values in mind as I applied. Thinking... -
Plasmids 101: Modular Cloning
TypeBlog Post...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...focused on developing cell-free synthetic biology applications for human health and the environment. Alexander...nanomaterials science, and self-assembly for applications in health, energy, and sustainability. References... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...the initial laser output. The same considerations apply to the polishing and preparation of the patch cable...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...and respond to novel substances. We both had an applied science bent, so the engineering aspect of this...about continuing with the business. We ended up applying to a business accelerator freely stating that ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...machine learning (PRIDICT, PRIDICT2.0). How to apply PRIDICT in prime editing experiments Prediction ... computational biology. His expertise includes applying machine learning techniques to make sense of biology... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...problem for fusion proteins. One of the major applications of FPs is to visualize the localization, dynamics...An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue ... -
Great Results Start with Great Standard Curves
TypeBlog Post... critical for a number of popular scientific applications, and the quality of a standard curve can make...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95. Additional... -
An Introduction to Adenovirus
TypeBlog Post...Persistent infection by adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136...on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting... -
Getting to Know Expansion Microscopy
TypeBlog Post...with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...