We narrowed to 442 results for: RELA

1. Isolating Genomic Regions of Interest with the CRISPR System

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   ...of the growth factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates...

2. Viral Vectors 101: The Retroviral Lifecycle

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   ...The genome of viral tools are often not virology related at all, and you will instead get expression of ...

3. Negative Can Be Positive: Open AAV Data with Addgene

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   ... AAV questions every year on a range of issues related to AAV vectors, many from people trying to answer...

4. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...

5. CRISPR 101: Making a Knock-In Cell Line

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   ...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...

6. AAVs in Retinal Gene Therapy

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   ...X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will ...

7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...

8. Lentiviral Vector Uses and Overview

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   ... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise...

9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ... necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid...

10. Cpf1: A New Tool for CRISPR Genome Editing

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    ...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...

11. A Career in Grants: How To Become a Grant Professional

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    ...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers....

12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ... of the reasons why we have only three biology-related Nobel prizes, out of which only one was done in...

13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...in what position do you want your gene to be in relation to the rest of the genes? And do you want it to...

14. CRISPR 101: Any Base Transversion Editors

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    ...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...

15. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...

16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...

17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1, but they require both a tracrRNA and crRNA...

18. To Codon Optimize or Not: That is the Question

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    ...studies have shown that translational efficiency is correlated with codon bias across all endogenous genes in...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E variant ...

20. Viral Vectors 101: Pseudotyping

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    ...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...