We narrowed to 1,006 results for: RING

...new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns and ... of chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon... Mary Gearing...

...DCL) process the dsRNA into specific types of interfering RNAs.   RNAi vs. CRISPR: Complementary techniques...fundamental steps: creation of the RNAi trigger delivering the RNAi trigger characterizing the RNAi phenotype...from a long hpRNA trigger, only a single ~21-nt interfering RNA is produced using shRNA/amiRNA. Although ...evaluated for their gene silencing efficacy, thereby requiring a substantial time investment. Once the target...widely implemented in plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang...Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing...

...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...organization what do you think of? Maybe the term brings to mind a social service organization like the ...believes there is strength in diversity and in empowering team members to take initiative and give their...our mission to accelerate research with easier sharing and collaboration. Joanne and all the Addgenies...

...enables researchers to silence or incite neuronal firing and study subsequent effects on behavior. The system...activation of light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit...the cell membrane when exposed to light. Neuronal firing depends on these charged particles and whether ...said to be hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the...functions as a neuron “off switch.” Because of the differing peak absorption wavelengths and light intensity...

... the importance of leaving room for serendipity during your PhD studies and offer two simple steps to ...you to discussions beyond your immediate field, fostering a diverse range of perspectives and sparking innovative...capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...How to Lead a Great Meeting Form Your Own Peer Mentoring Group...

... project. Dissemination of the knowledge gained during this period occurs infrequently and rarely makes... wondered how we could make the insights gained during this time more visible and thereby have a positive...reviewers of your expertise and insight. We recommend sharing ReFigures on your Linkedin and ResearchGate profile...Please support ReFigure by creating ReFigures and answering this 2 minute survey! You can also find ReFigure...science at We Share Science Learn more about Data Sharing Resources at Addgene.org Deposit Your Plasmids...

...used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in Pamela Silver’...enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly think that...and interesting than that will simply get people ordering plasmids. The need to keep content coming also...that I thought about careers outside of academia during my graduate training. PhDs have many roles to play...different fields. If you start thinking about preparing yourself for a career while still in graduate ...

...my recommendations on some ways to get started sharing your science with the community. Some of them you... up your own twitter feed or talking about and sharing some science on the one you already have. Read ...University. At Addgene we're committed to scientific sharing – both your plasmids and your research stories....  sharing your science... scientific sharing...

...on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s focus on how to ...School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor is a good...have good observations and be less inhibited in sharing concerns. Ask alumni of the lab. Most labs have...Depace's Talk on "Precision and Plasticity in Mentoring" Don’t ignore warning signs If lab members, neighbors...a Good Mentor?" and 6 More FAQs About Science Mentoring ...

...dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent affinity...target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids...norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...peer-review is a touchstone of the scientific method, ensuring that published work meets the accepted standards...or omission from the literature. I think that delivering constructive critique can help your colleagues...following morning - as usually deadlines are pressing. During this second read, I pay more attention to details...passionate about open science, BIG data analysis and sharing whatever she knows with whoever cares to listen...the Addgene blog Find blog posts on scientific sharing Get tips on writing a review article Resources...

...gene expression have similar drawbacks, often requiring the destruction of plants or tissue for GUS staining...show the effects of the mutation. But ¼ of the offspring from these plants will be green, containing the...removed to prevent further editing, then green offspring can be selected instead of red ones. Other innovative..., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic... resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

...lamenting the viral production process while still ordering viral vectors. Virus production can be a frustrating...experiments they’re interested in faster by producing, titering, and testing viral preps for them. At the same... service: "I'm excited that Addgene is now offering virion preparations to the scientific community...involving CRISPR and so we also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on...continue to develop it. More communication and sharing will only help research advance. Remember: productivity...

...our eyes open for any new technologies that will bring about the next revolution in biological research...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog, Cpf1, may make it easier...difficult to use with CRISPR as the cargo. Mary Gearing explains some of the clever ways to successfully...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...

...requires an immense amount of self-knowledge and an unwavering devotion to seeing your ideas become something...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab...generation companies.  We launched in the early spring of 2015 and since that time have received about..., including one led by a talented biomedical engineering team from MIT.   I would like to share my own... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials...

...advancements include work exploring far-red-emitting dyes in bacterial imaging, offering solutions to overcome...Photostability, the ability to retain fluorescent intensity during exposure to light, is another factor to consider...wavelengths, as this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation...facilitate clear differentiation between multiple dyes during analysis. Image resolution is impacted by several...Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield...

... 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase ... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...acceptor molecules are targeted nearby (such as a neighboring house on the same street as the donor), then ...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling calibrated FRET distance...

... when scientists bring biases to research and don't collaborate with others to bring diverse perspectives... rules all, the books center around a cast of endearing characters who decide to take matters into their...dueling magicians in the 1800s in England who help bring back actual magic (which had been lost for an age...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...

...roles in fluorescent protein development by openly sharing their resources with the research community. To...protein abundance, possibly due to misfolding. But measuring variants one at a time is time consuming and unscalable...use the sensor to answer the question of whether firing of serotonergic neurons (measured using a red calcium...sensor in sleep-related studies and in assays for measuring human serotonin transporter activity. Unger also...dynamics with the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously...

...a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles...released from the producer cells into the supernatant during the production phase. However, this has not been...at the AAV vectors that are released from cells during production (Maguire et al., 2012). They saw that...: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells using ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...