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We narrowed to 622 results for: SET

Showing: 361 - 380 of 622 results
  1. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...
  2. Bioinformatics at Addgene

    Type
    Blog Post
    ...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...
  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...
  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...
  5. Hot Plasmids - August 2020

    Type
    Blog Post
    ...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...
  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...
  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antigen or an antibody depending on how the assay is set up. Then they block the capture reagent to prevent...binding to a primary antibody.  While the precise setup of the method can vary, typically users first coat...
  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...facilitates the use of prime editing in pooled settings with hundreds to thousands of pegRNAs. Chromatin-based...primer binding site, and many more. Once we had our datasets with all the different features and editing efficiencies...PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in the training of PRIDICT2.0 make this model...
  9. MXS Chaining

    Type
    Blog Post
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...
  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...and/or perceived professional risk involved in setting up a CURE, especially if it’s a new approach within... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...
  11. CRISPR Antimicrobials

    Type
    Blog Post
    .... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...
  12. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...
  13. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...
Showing: 361 - 380 of 622 results