We narrowed to 556 results for: SPR

1. RaPID Detection of RNA-protein Interactions

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   ...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...

2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence...Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation...

3. Plasmid Tools for Microbiome Studies

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   ...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

5. Celebrating 15 Years of Scientific Sharing

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   ...our “google doc of gratitude.” We must keep spreading the word about materials sharing, but we don’t... who support us by depositing, requesting and spreading the word. Our blog and site resources are just...

6. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...happening within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see ... contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

8. Viral Vectors 101: An Introduction to AAV

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   ...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...

9. Lab Automation at Addgene

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   ... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of interest may be very ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

11. Fluorescent Proteins 101: Introduction to FRET

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    ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ... of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with...Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

14. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource Center ...Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources Deposit Your Drosophila Plasmids ...

16. Experimenting with New Careers while in Grad School

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    ...the community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to make a trophy...

17. Luciferase Plasmid Collection

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    ...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV9-CRISPR knockout plasmid. Renilla luciferase is used to...Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout plasmid. Firefly luciferase is used to...transfection Sidi Chen 109396 TBG-Vector Firefly TBG AAV-CRISPR knockout plasmid. Firefly luciferase is used to...

18. AAVs in Retinal Gene Therapy

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    ...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ...

19. Tips for arabidopsis transformation

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    ... (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https://...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...

20. Course-Based Undergraduate Research Experiences (CUREs)

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    ...history. Interestingly, he also adds to the disproportionately large number of honorees to have trained ...Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects MDH-focused CUREs at...