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Showing: 361 - 380 of 711 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    ... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are amplifying...taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... mechanism for this function is not yet fully understood, we can imagine that this transport is probably... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles...

  4. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

  11. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much...

  12. Science Career Options

    Type
    Blog Post
    ...are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant Breeder ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we are providing lentiviral...packaging plasmids to create your own lentivirus stocks. Our research scientists have provided their standard...

  14. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...

  15. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ...
Showing: 361 - 380 of 711 results