We narrowed to 718 results for: STO;

1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   ...were many excellent applicants but Xin and Fatima stood out for their creativity in the use of fluorescent...shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   ...first post here. Once you’ve identified some fly stocks and other reagents of interest, the next question...regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety...

3. 5 Great Apps for Lab Life

   Type

   Blog Post

   ...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain...

4. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   ...including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive...

5. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   ...Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. For your...until you have good separation between your bands. Stopping the gel when the bromophenol blue dye line is ...

6. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...the frozen cell line becomes non-recoverable, the stock of the mAb becomes limited, which can be a threat...able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the cells. This RNA ...

7. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   ...Great! But don’t just stop using it cold turkey. Step back gradually; maybe you stop to check things off...

8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   ...status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by...

9. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance...would like to thank all of our depositors and requestors for making their reagents available and for supporting...

10. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    ...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below!   Additional resources ...

11. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    ...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic...

12. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...

13. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    ... tasksDelegation can feel unnatural for those accustomed to hands-on work. The temptation to "do it yourself...Matteo Tardelli authored the book "Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry...

14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    ...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ...

15. A Deep Dive into BioBuilder

    Type

    Blog Post

    ...space offers STEM programming, workshops, and customized programs to meet the needs of students and teachers...blogger, Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work...

16. How Addgene Creates Content

    Type

    Blog Post

    ... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ...

17. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...involved in central anthocyanin metabolism are well understood. Additional work has also identified and characterized...how you were able to overcome them. Share your stories in the comments below! And if you have developed...

18. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...

19. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    ...neurons whose function and connectivity are poorly understood,” says Qiu.   Qiu developed a dual virus system... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide...