We narrowed to 728 results for: STO;
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TypeBlog Post...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out...
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Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...were many excellent applicants but Xin and Fatima stood out for their creativity in the use of fluorescent...shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...first post here. Once you’ve identified some fly stocks and other reagents of interest, the next question...regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety... -
Addgene Depositors Get More Citations
TypeBlog Post... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much... -
Science Career Options
TypeBlog Post...are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant Breeder ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we are providing lentiviral...packaging plasmids to create your own lentivirus stocks. Our research scientists have provided their standard... -
5 Great Apps for Lab Life
TypeBlog Post...devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps...acids memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. For your...until you have good separation between your bands. Stopping the gel when the bromophenol blue dye line is ... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance...would like to thank all of our depositors and requestors for making their reagents available and for supporting... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below! Additional resources ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...calcium events), Greater brightness, Less signal distortion from photoswitching or photobleaching Improved...addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post... tasksDelegation can feel unnatural for those accustomed to hands-on work. The temptation to "do it yourself...Matteo Tardelli authored the book "Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry... -
How Addgene Creates Content
TypeBlog Post... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...the frozen cell line becomes non-recoverable, the stock of the mAb becomes limited, which can be a threat...able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the cells. This RNA ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...Great! But don’t just stop using it cold turkey. Step back gradually; maybe you stop to check things off... -
A Deep Dive into BioBuilder
TypeBlog Post...space offers STEM programming, workshops, and customized programs to meet the needs of students and teachers...blogger, Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work...