We narrowed to 452 results for: SUP

1. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of...

2. Transferable Skills: Negotiation

   Type

   Blog Post

   ...strengthen relationships with your colleagues and supervisors and lead to you being remembered as a person ...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...They are isolated mostly from abnormal and/or supernumerary preimplantation embryos that are produced during...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...PEG precipitation of the HEK293 cell culture supernatant because it’s time consuming to prepare the 40%...

5. Magnetic Control of Proteins: More than a Dream

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   ...frequent collaborator and is happy to give advice and support for follow-up studies. “We don’t have a vision ...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...sized Lambda amplicon inserts to control for any suppression of background by antibiotic selection. Screening...

7. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ...optimize. One reason could be the instability of the supposedly alien 5’-phosphorylated ssDNA guides, which might...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

   ...monomeric FPs. EGFP and other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise,...

9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by...

10. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ... These parts have been carefully engineered to support reliable functional composition. In other words...

12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins...

13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This...

14. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...LwaCas13a, which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In ...

15. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...successful scientists have outgoing personalities to supplement their intellectual prowess and scientific skills...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...requests. Afterwards, from 2/24/20–3/15/20 (the supposed end of lockdown), request activity appears to ...

17. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...notice typically should include the paper, any supplemental data, software repositories, or other information...

18. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ... let us know! Contact our scientific technical support at [email protected] and let us help.  Check back...

19. AAVs in Retinal Gene Therapy

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    Blog Post

    ... large scale manufacturing of viral vectors to support trials for prevalent diseases - to name a few. ...

20. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...stable expression of the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis...