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We narrowed to 609 results for: She

Showing: 361 - 380 of 609 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the... including the development of crops with longer shelf life and the development of disease-resistant crops...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...offended. I felt terrible when the host told me and wished I had been more careful. 3. Tell a story We are...thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time ...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...read a paper and saw an interesting construct you wished to use, you could either make it yourself or you...Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release with an optogenetic...

  6. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the presence...treatment resulted in rapid internalization and diminished luminescence signal, associated with endosomal...

  7. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional...Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression with the Q repressible...

  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...  Once you have chosen an antibody read the data sheet thoroughly. Vendors will frequently include suggestions...proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab doing experiments...was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on getting results...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

  16. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue culture treated dish, ...trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate the independent...

  17. Kit Free RNA Extraction

    Type
    Protocol
    ...opens in a new window) TRIzol® User Guide from ThermoFisher Scientific . Figure 1: A diagram of the different... Caution Make sure to read the SDS (Safety Data Sheet) for safety warnings and hazards for these reagents...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...) Opti-MEM, Thermo Fisher 31985-070 0.05% Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue culture treated dish, VWR,...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250...
Showing: 361 - 380 of 609 results