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  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional...Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression with the Q repressible...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...  Once you have chosen an antibody read the data sheet thoroughly. Vendors will frequently include suggestions...proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    ...tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab doing experiments...was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on getting results...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

  7. Pipetting Protocol

    Type
    Protocol
    ...pipette, and the tip ejector ring at the bottom which pushes the pipette tip off of the pipette. This side view...rate into the pipette tip. Once the liquid has finished drawing into the pipette tip, lift the pipette...

  8. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium salt, Sigma Aldrich... Grade, VWR 75800-188 10% Pluronic F-18, Thermo Fisher 24040032 Glutagro, Corning 25-015-CI BalanCD HEK293...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...

  12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue culture treated dish, ...trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate the independent...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...opens in a new window) TRIzol® User Guide from ThermoFisher Scientific . Figure 1: A diagram of the different... Caution Make sure to read the SDS (Safety Data Sheet) for safety warnings and hazards for these reagents...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...) Opti-MEM, Thermo Fisher 31985-070 0.05% Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH...

  15. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue culture treated dish, VWR,...of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...advantage of AAV vectors is that there are well-established protocols for their production and purification...PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur, E.L., and Wolfe...
Showing: 361 - 380 of 596 results