Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 561 results for: Spr;

Showing: 361 - 380 of 561 results

  4. Lab Automation at Addgene

    Type
    Blog Post
    ... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...

  5. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al..... Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

  11. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...the best promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments...NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...

  12. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...history. Interestingly, he also adds to the disproportionately large number of honorees to have trained ...Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects MDH-focused CUREs at...

  13. Tips for arabidopsis transformation

    Type
    Blog Post
    ... (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https://...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...later. The PNS presents a different problem: it’s spread throughout the body and is often difficult to surgically...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library...

  17. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful ...Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins. ...Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins. ...Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human transmembrane ...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  20. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...
Showing: 361 - 380 of 561 results