...Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org...Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

...needs to be added to media after autoclaving. If spread on top of pre-made plates, make sure it is evenly...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...

...Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence...Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation...

...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

...our “google doc of gratitude.” We must keep spreading the word about materials sharing, but we don’t... who support us by depositing, requesting and spreading the word. Our blog and site resources are just...

...happening within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see ... contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes...

... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...

... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...

... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

...intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of interest may be very ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma...

... of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with...Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource Center ...Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources Deposit Your Drosophila Plasmids ...

... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

...the community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to make a trophy...

...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ...

...later. The PNS presents a different problem: it’s spread throughout the body and is often difficult to surgically...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...