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We narrowed to 927 results for: TIM;

Showing: 361 - 380 of 927 results

  1. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...they've managed to keep themselves happy during their time in grad school. As you'll learn, grad school has...topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance...right that illustrate a grad student's happiness v time throughout graduate school. This is, of course, ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

  2. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...hear that we've been able to help their labs save time and resources by storing and distributing their ...about making such tools open-access. Though it is time for me to refill my large coffee cup and get back... – so if you’re running out of energy, then it’s time for a coffee run! ...

  3. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune...Zimmer’s coverage on the subject in the New York Times and on his blog, where he has offered to answer ...

  4. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...organisms and their characteristic radiance for some time – there are records describing them dating back ...back to the first century A.D. – the first time anyone truly examined what caused this glowing phenomenon...organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work, scientists really started...fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...systems, the individual parts are reusable, saving time and money on future assemblies. If you are new to...for eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech...guides, mini screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries...parts can be used in a variety of organisms, saving time and money for labs with diverse applications. The...already in place. MoClo is a fantastic way to save time and money on studies that require many assemblies...

  6. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...sample. As more than one hole is illuminated at a time, the image can be acquired more quickly. 2-Photon...single focal plane of the sample is excited at one time, limiting photobleaching. Similar to widefield, ..., the entire field of view is excited at one time and captured in a single camera exposure. This is much...technology and parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial...

  7. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...importantly, there’s the opportunity cost of your time; an investment of months of your life that may end...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...way to do something is to do it huge, there are times when a more incremental approach is prudent. What's...      References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

  8. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we... others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly...is that if you introduce them into cells one at a time, you could identify those that confer resistance...

  9. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing...using one of these validated gRNAs can save you the time that would be spent making and testing entirely ...CRISPR experiment.  The selection process is often time consuming and there is no guarantee that the selected...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine whether...

  10. Science as Art

    Type
    Blog Post
    ...skill,  time for which most researchers wouldn't have. They are motivated to get data, publish in time, defend... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...its stunning images that is has been featured in Time magazine . Other smaller competitions are now taking...

  11. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier...been slower to adapt, likely due to the necessary time and resource investment required to change the status...’ll be able to make updates to protocols in real time. You may also want to consider moving your paper...added or removed samples. Once you've taken some time to get organized, stop back and let us know what...

  12. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... stage, before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for ...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies...

  13. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

  14. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...account for three years and have only tweeted 6 times: #fail. I’d hoped to use Twitter professionally ...scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top 20 Domains Tweeted by Scientists... contain scientific domains between 0-10% of the time; however, ~20% of life scientists  (biologists, ...ecologists) tweet scientific domains 20% or more of the time (red box). 8. Scientists tend to follow other scientists...

  15. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...took the time to answer our questions.   Advice for success in academia How can we shape our time in academia...passion. “Love what you are doing!” It helps when the time comes to work with full force. George Church 1....during their studies. They suggest that you make time for extracurricular activities both to develop new...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...exceeding a kit’s capacity can actually reduce your ultimate plasmid yield.  Take your average column-based...available for plasmid purification. Kits can be optimized based on the plasmid size, cell model, or overall...size. Some manufacturers offer kits specifically optimized for purification of these large plasmids, while...and for extracting RNA! (That’s a blog for another time, however!) Where can you look for help?  All manufacturers...Different Quantities, Different Uses Plasmids 101: Optimizing Plasmid Yields Bringing Sustainable Practices...

  17. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you...until the article is published. This policy gives us time to complete necessary quality control and legal ...distribution, and we’ve found that this step was sometimes unclear or missed altogether. Emailing Addgene...citation to the final journal article version any time later. We recognize that a preprint isn’t necessarily...

  18. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...with a master’s. At the time my degree felt like a consolation prize, but in time I realized its value. ...helped develop my science communication skills. Ultimately, my degree prepared me to be exactly the type...1. Are you interested in master’s-level jobs? Ultimately a degree is a means to gain employment, so it...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...an entire classification scheme, known as the Baltimore replication classes, has been developed around...around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...And now that you know what viral vectors are, it’s time to dive into how they’re used! We have a growing...

  20. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...outstanding material transfer agreement (MTA) turnaround times: less than two days for material requests and less...agreement must be put in place. And subsequently, every time that material is requested, a material transfer ...By default, your TTO must sign an agreement each time a material is being requested or deposited. But,...future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to basically...
Showing: 361 - 380 of 927 results