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We narrowed to 936 results for: TIM;

Showing: 361 - 380 of 936 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...working on one process at a time, and testing a small set of data each time a new process was added, we...connected, as this enabled us to rapidly iterate and optimize our pipeline. After reviewing and testing several...path forward was clear: to build one process at a time and connect each subsequent process to the existing... the compute resources when needed, for faster runtime and decreased cost compared to purchasing and maintaining...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...Church. "Genetically encoded sensors enable real-time observation of metabolite production." Proceedings... as most translation initiation sequences are optimised for a particular system. The Alexandrov lab at...specific alleles in microbial populations through time, Christopher Marx's lab has engineered and validated...quantitatively determine allele frequencies across timepoints or populations. This kit consists of a 48 plasmid...reveals an ordered progression to resistance." Antimicrobial agents and chemotherapy 57.11 (2013): 5373-5383...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...restriction sites (or lack thereof) nor a serious time commitment. Another advantage is that this process...generate wild type and mutant constructs at the same time, rather than sequentially.   How does Gibson assembly...screen a large amount of colonies. Excluding the time it takes to get primers, you can have your construct...their manufacturers tout great efficiency and low time commitments. These kits also come with specific ...such as increased efficiency, shorter incubation times, or the ability to accommodate smaller fragments...

  4. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...regular post-holiday routines, why not take a little time to appreciate the humor in science and in the lab... all a place that encourages people to take some time out every once in a while – just read Melina Fan... people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, of course... you that laughter really is a great use of your time even as that lab bench is calling, let me share ...

  5. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each plasmid...others to ensure that everything will ship out in time - from assembling the boxes, to inoculating tubes...scale reagent sharing led us to calculate how much time we have saved researchers. In a recent blog post...

  6. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis. Listen ...scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's... opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...structural and functional optimization to increase the specificity and stability. Optimizing antibodies for better...efficient and targeted delivery of treatments, ultimately increasing treatment effectiveness. How do you.... How you engineer an antibody depends on your ultimate goal, and some engineering projects will be easier...experiments, you’ll typically go through this cycle many times, until you achieve whatever goal you’re aiming for...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...serotypes may perform better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2...outlined in the table below. Cell Type Cortex Optimal Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 ...quickly. 3. Patience Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV ...see that gene expression really does not take much time at all. Much of the previous literature using AAVs...3 days vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use AAV constructs...

  9. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...they've managed to keep themselves happy during their time in grad school. As you'll learn, grad school has...topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance...right that illustrate a grad student's happiness v time throughout graduate school. This is, of course, ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

  10. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...hear that we've been able to help their labs save time and resources by storing and distributing their ...about making such tools open-access. Though it is time for me to refill my large coffee cup and get back... – so if you’re running out of energy, then it’s time for a coffee run! ...

  11. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune...Zimmer’s coverage on the subject in the New York Times and on his blog, where he has offered to answer ...

  12. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...organisms and their characteristic radiance for some time – there are records describing them dating back ...back to the first century A.D. – the first time anyone truly examined what caused this glowing phenomenon...organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work, scientists really started...fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although...

  13. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...sample. As more than one hole is illuminated at a time, the image can be acquired more quickly. 2-Photon...single focal plane of the sample is excited at one time, limiting photobleaching. Similar to widefield, ..., the entire field of view is excited at one time and captured in a single camera exposure. This is much...technology and parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...systems, the individual parts are reusable, saving time and money on future assemblies. If you are new to...for eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech...guides, mini screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries...parts can be used in a variety of organisms, saving time and money for labs with diverse applications. The...already in place. MoClo is a fantastic way to save time and money on studies that require many assemblies...

  15. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...importantly, there’s the opportunity cost of your time; an investment of months of your life that may end...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...way to do something is to do it huge, there are times when a more incremental approach is prudent. What's...      References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

  16. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we... others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly...is that if you introduce them into cells one at a time, you could identify those that confer resistance...

  17. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing...using one of these validated gRNAs can save you the time that would be spent making and testing entirely ...CRISPR experiment.  The selection process is often time consuming and there is no guarantee that the selected...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine whether...

  18. Science as Art

    Type
    Blog Post
    ...skill,  time for which most researchers wouldn't have. They are motivated to get data, publish in time, defend... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...its stunning images that is has been featured in Time magazine . Other smaller competitions are now taking...

  19. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier...been slower to adapt, likely due to the necessary time and resource investment required to change the status...’ll be able to make updates to protocols in real time. You may also want to consider moving your paper...added or removed samples. Once you've taken some time to get organized, stop back and let us know what...

  20. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... stage, before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for ...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies...
Showing: 361 - 380 of 936 results