Hot Plasmids - February 2022
Type
Blog Post
..., including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...
We narrowed to 508 results for: Trac
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Sweating the Small Stuff: Details in the Lab
TypeBlog Post...pipette tips to match your plate layout can help you track samples as you add them. Moving your reagents from... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...This information is important, and you’ll need to track it throughout your experiments. Now that that’s... -
Antibodies 101: Isotypes
TypeBlog Post...which can initiate a cascade that helps attack extracellular pathogens. Transportation of antibodies to ... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post... your advantage As the profession gains more traction in the sciences, I believe that more and more ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post... it doesn’t work in many cases. In an effort to track down every last one of those gene regulatory proteins... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...improvement in activation and knew she was on the right track. The next insight was to assemble a synthetic transcription... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...their idea at various levels of complexity and abstraction and convince people with diverse backgrounds ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...required? CasPEDIA provides information on crRNA and tracrRNA requirements, along with any other required non-CRISPR-associated... -
Viral Vectors 101: Types of viruses
TypeBlog Post...replicate episomally, essentially remaining an extrachromosomal part of the host genome that can replicate... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...and regular check-ins, which helped them stay on track and feel supported. Another team member was highly... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post... of shRNA in target cells by the addition of tetracycline/doxycycline to the grown media resulting in ... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...live with is aware of the event to limit all distraction. On the day of the interview, dress the part,... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...with input from the group to make the plan better. Track your goal progress as a group. Practice your job... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...this, the phosphorylation status of MEK1/2 was tracked at 5 minute intervals via Western blot. Phosphorylation... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...experiments. These background proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...sucked to work remotely. I was also tired of the contracting life where you do a little bit of work for one... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...about a third of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The...