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We narrowed to 770 results for: VARS

Showing: 361 - 380 of 770 results

  1. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...lacZ reporter gene (3, 4), there are now many variations, including many that use auxotrophic rescue genes...provides a baseline level of reporter activation. Vary the expression levels of bait and prey proteins ...your protein are screened for binding. Using a variety of expression levels and systems can alleviate ...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out... people outside the department to accomplish a variety of goals. For example, I worked with some microscopy...current job at Addgene I work with people from various organizations in science and beyond. For example...

  3. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... knowledge they require. Biotechnology companies vary to what extent they hire experts for their technical...complain about high background. In many instances, variations of the product’s specific application instructions...support roles A job in technical support can add a variety of skills to your CV and lead to other job opportunities...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene we're helping...5 mental mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from...brief exercise to spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools... efficiency, purity, and indel rate of each tool vary considerably. And there’s so much more exciting ...models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...use Gateway to clone a gene of interest into a variety of expression systems. Keep reading to learn more...ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the...Addgene. These can be used to express genes in a variety of model organisms. Use the links below to find...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most of) MIT and the University...sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department of Brain and Cognitive...Boston Children’s Hospital is the only part of the Harvard group that has responded to the FLSA at the time...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...mouse lines that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...cell lines can become cross contaminated and are invariably much older than those available from biorepositories...thaw a new batch. The exact number of passages can vary between cells lines, as this can be dependent on...culture research experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...

  11. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...

  12. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...pragmatic adoption (although it might be argued various authors have simply chosen to submit elsewhere)...effort. Our standards of data recording are highly variable and usually left to individual researchers. Yet... said to be representative or the statistical variation is calculated and placed in the legend. Widely...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...constantly evolving and demanding field, requiring a variety of biological and molecular tools. One such tool... Arabidopsis include understanding of allelic variation between different plant species and metabolic ... Various Addgenies...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose from and can be catered...translation machinery. The composition of the RISC varies, but at the very minimum requires an Argonaute ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...

  15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh et al., 2014)...that expression of your reference protein does not vary under your experimental conditions. Otherwise, you...control won’t be strongly influenced by unexpected variations in any individual protein. This ensures you are...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...splenocytes, you might only be interested in one of the various cell populations; for example, CD8+ T (“T killer...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...processed piece of tissue, where cells are present that vary in cell type, size, and marker proteins. But even...parameters the machine uses to differentiate between variations in those factors. To understand the principles...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

  18. Five Popular Model Organisms

    Type
    Blog Post
    ...have successfully helped researchers develop a variety of therapies. For example, many scientists use ...and easy to work with as they can survive in a variety of environmental conditions, and double every 2... Various Addgenies...

  19. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al, 2018; Cranfill...linker length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    ...Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell phones, and...accurately. We're sure to see many improvements in a variety of biological technologies as both time and research...Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the serotonergic ...
Showing: 361 - 380 of 770 results