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...activate transcription by causing conformation changes in the AraC dimer. In doing so, the dimer shifts...activation is.   Figure 2: A bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph ... Jennifer Tsang...

...ahead of time included contacting YNP staff to arrange permits to visit places not accessible to the public... around these hot springs make them extremely dangerous, we used telescoping poles to collect water samples...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite...

...study it and see what happens when technological change happens really fast,” Thompson says. To do this... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors... Jennifer Tsang...

...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van Haren ...and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast...

...nice to have, not a need to have Must be ready to change projects frequently with little notice or choice...someone else who can do better, no grant writing Job changes can happen regularly (every 4-6 years is common... create interpersonal issues (one bad apple can change entire culture) Specific requirements for hiring...

...References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided genome editing...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ...

...devotion to seeing your ideas become something tangible.  When you think of entrepreneurship in the 21st...company’s development  Don’t be afraid to email strangers asking to meet. I have cold emailed hundreds of...including Harvard/MIT faculty, venture capital and angel investors, and business development heads at major...

...past the chatting stage you will rarely make any change in your skill sets or activate the helpful accountability...of working with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring...for Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

... tremendously complicated entities with a large range of characteristics (color, brightness, photostability...FPbase.org FPbase is a project written in Python & Django, using a popular database management system (PostgreSQL...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ...

...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury.... diverse groups of scientists working in a wide range of research fields and geographical locations. In...

...This guest post was contributed by Ally Huang is a 4th year PhD student at MIT. While I had always enjoyed...make learning biology more fun – bit by bit. Ally Huang is a 4th year PhD student at MIT in Biological Engineering... can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic...

...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...

...MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas ...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...School of Management. His research focuses on a range of topics including biotech and research tools, ...

...can feel lost. Use this post to explore the wide range of careers available to scientists and open your...carry out in-depth research is applicable to a wide range of industries and is a highly desirable quality ...SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science Reporter Technical...

...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting...recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed PMID: 28288123... also provided a detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure...flame depositor David Liu’s lab increase both the range and specificity of base editing. Kim et al. used...

...these reporters, editing typically results in a change in the fluorescent readout. In this blog we will...for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells.  A...GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did not surpass...

...related plasmids in our collection, we’ve relied on Sanger sequencing as a first pass QC. The lab team manually...that process is complete, QC scientists prepare Sanger reactions by mixing this isolated plasmid DNA with...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours...

...bacteria out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which... of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures,...discussions. Not only are such endeavors a welcome changeover from conventional academic settings, these are...

...enzymes function across a much broader temperature range than BioID, which only functions optimally at 37... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...