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We narrowed to 466 results for: organization Addgene

Showing: 361 - 380 of 466 results

  1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment Much like FRET.../10.2144/000113943 Additional resources on the Addgene blog Browse our Fluorescent Protein 101 blog posts...covering an introduction to FRET Resources on Addgene.org Find fluorescent protein plasmids and resources...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Central PMCID: 3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs ...AAV, and much more! Find ready-to-use AAV from Addgene here or check out these protocols if you want to...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution and has...[K28/43R] under the Application section of the Addgene antibody page, and learn more about the whole-brain...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance Plasmids...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Resources and References More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements ...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus... This blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  7. MXS Chaining

    Type
    Blog Post
    ...Resources on Addgene.org Browse our Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The...and BamHI EcoRI Additional Resources on the Addgene Blog Read about Gibson Assembly Learn About Other... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...it's impact on human health.     Resources on the Addgene Blog Learn How to Experiment with New Careers ...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...including the age of the model organism, injection site, and titer. Addgene’s Viral Vector Service Besides...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...quality of the prep. This was a large factor in Addgene’s decision to launch our viral vector service in...able to order high quality viral vectors from the Addgene repository can save researchers significant time...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...structures or organelles, such as remodeling the cytoskeleton or blocking membrane trafficking. Addgene has assembled... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Do you have suggestions...localization patterns for colocalization studies or organelle labeling. Plasmid... fusion proteins that are targeted to various organelles or subcellular structures. To compare with a ...Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... as an ideal target. It is an easily accessible organ that can be monitored non-invasively over time. ...PubMed PMID: 26958729.  Additional Resources on the Addgene Blog Adeno-Associated Virus (AAV) for cell and...for gene expression in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  12. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...Addgene recently moved to our brand new location, with more space to grow and help scientists share resources...Gene names! This is not surprising for Addgene. But the names we chose were not just any gene names. Now,...gene gets its name based on what happens to an organism when a gene/protein is depleted in a knockout ...embryonic development The largest conference room at Addgene is named Sonic Hedgehog but this is not a direct...divide properly into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can ...the stomachs of different animals. Check out the Addgene website for plasmids that contain tinman or the...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the wide array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect...variety of species and cell types can be found on Addgene’s website. Activating target genes using dCas9-based...variety of species and cell types can be found on Addgene’s website. Fig. 3: Cas9 repressors include...What do i do next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that...drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on the various delivery... ). 368, 290–296 (2020). More resources on the Addgene blog Read all the posts in our CRISPR 101 Series...

  14. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...suitable for organoid or in vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...

  15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...al  2017). Find Additional CRISPR Resources on Addgene's CRISPR Guide Page 2. Optimizing "cut-to-mutation...PubMed PMID: 28102837. Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How...Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed... (2017). PubMed.  Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...provide value to others This is a non-profit organization. Therefore, collaboration and community is the...Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental Designs Consulting...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

  20. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...vectors (for a more detailed explanation, check out Addgene's Lentiviral Guide). To produce lentiviral vectors...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation... how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...Central PMCID: PMC9377332 More resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle...every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References and resources... to All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors...
Showing: 361 - 380 of 466 results