We narrowed to 616 results for: set

...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...

...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...it’s simple. The RT protein is made by a separate set of instructions in the same producer cells as the...

...and/or perceived professional risk involved in setting up a CURE, especially if it’s a new approach within... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...

...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...

...that do not express the protein. With this control set, you should see your target protein IP’d in the positive...

...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

...sales-y”. The work includes reviewing customer data sets, troubleshooting protocols, analyzing information...

...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...

...discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority of the ...

.... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

...example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with...

...Fridays. From each overnight culture, we prepare three sets of 2D barcoded tubes. Two of the tubes are used ...

...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

...MfeI. With oligo overlap cloning, you can design a set of oligos containing your desired restriction sites...

...technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility...

... (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our...

...team members only, under the specific permissions set for every member of your team. Do you need to make...