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  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...
  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... populations of synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4...immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry (MS) in HCT116 cells (colorectal cancer). The...isolated and identified with tools like mass spectrometry, providing a snapshot of interacting proteins...
  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...
  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...
  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...
  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... et. al. Science . 1996 272:263-267. PubMed . Asymmetry in the assembly of the RNAi enzyme complex. . ...Bioanalytical catalog # AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar ...
  7. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...
  8. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... if an export license is not required for your country after checkout, and the processing fee will be ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...
  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...date, material, requesting PI & organization, and country. This information can be of personal interest to...horizontal bar chart) Number of requests per country (as dot plot) Number of requests per year (as...
  10. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition, and...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...
  11. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ... data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme...factor, study topic, and the corresponding author's country of origin, were taken into account. 7)     Science...
  12. An Addgene Summer Internship

    Type
    Blog Post
    ...was unsure whether or not I wanted to major in biochemistry. There, I had exchanged contact with Amy C.,...internship at Addgene. They plan on majoring in biochemistry in college and play the drum set as their personal...
  13. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...can directly interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane...budget constraints Academic science is much like industry but the “product” is publications—the pressure...
  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...
  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...ranged from wildlife conservation to abortion law to poetry. A research discipline in its own right, science...
Showing: 361 - 380 of 529 results