We narrowed to 100 results for: EXO

...that, let’s take a look at exosomes. Proficient in transportation, exosomes are tools of communication...after injecting breast cancer cells into mice, exosomes expressed specific integrins (cell-adhesion proteins...proteins) targeting different organs. This means that exosomes not only promote microenvironment’s malignancy...also incite different organs to become malignant. Exosomes also contain miRNA (micro RNA) and other molecules...epigenetic changes in targeted cells. In this case, exosomes may transport miRNA from drug-resistant cells ...PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine organotropic metastasis." Nature...

...to manipulate RNA species through exon skipping. Previous CRISPR exon-skipping approaches used two guide...guide RNAs to remove a given exon from the genome, and showed success in models of muscular dystrophy. In...delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor and two putative splice enhancers...to iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio of pathogenic to non-pathogenic...

...The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity can be thwarted...available to polymerize, allowing the slower exonuclease activity to proceed. By including only one type...researcher can decide exactly how far the T4 exonuclease proceeds: at the first occurrence of the chosen...overhangs must not contain the dNTP present in the exonuclease reaction or polymerization will occur at that...

...the control happening? Is the regulation of an exogenously introduced gene or over the native locus? Let...knocked in flanking a gene of interest (or a single exon) and everything in between those sites can be excised... systems work by tagging the endogenously or exogenously expressed protein with the relevant tag and then... These systems can be easily implemented for exogenously introduced proteins, as many of them just need...

...First, it uses a dedicated 5’ exonuclease instead of using the exonuclease feature of T4 DNA polymerase... which contains three different enzymes: an exonuclease, which chews back the 5’ ends of the fragment...over 200 nucleotides. This may be because the exonuclease could chew through an entire fragment shorter...

...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose...β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation in ...in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation in subunit...

...result, gene silencing can be easily induced by exogenous application of long or short dsRNA. However, it...unmatched—unlike CRISPR experiments that require exogenous protein, successful induction of RNAi only requires... can maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon...microRNAs found in the genome, whereas shRNAs are exogenously derived. Importantly, instead of the assortment...

...promoter-less reporter which needs to integrate within an exon of a gene to generate a transcriptional fusion. ...do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt the gene...While this system does not require insertion into an exon, like promoter traps, it will still likely disrupt...

...paralogs. The exon-deletion hybrid gRNA library deletes human alternative cassette exons and disrupts ...disrupts genes with alternative exons. Get the library from Addgene New from the viral service By Jason Nasse...

...mutate the transcription start site, splice sites, exons, and/or promoters to knock out lncRNA function (...result in the retention of introns or the removal of exons. Disrupting promoter or transcription start site...(B) Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in: insertion...