We narrowed to 3 results for: EXO

...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity... thus does not need restriction enzymes or an exogenous ligase, providing an incredibly quick and easy...of three common molecular biology enzymes: 5' exonuclease, polymerase, and ligase. In Gibson assembly, ...together in one isothermal reaction. First, the 5’ exonuclease chews back the 5’ ends of your DNA fragments,...polymerase then closes the gap created by the 5’ exonuclease. Finally, ligase seals the nicks in the DNA to...potential to be completely degraded by the 5' exonuclease. Read more in our Gibson Assembly blog post ....T4 polymerase will continue to function as an exonuclease until a base is exposed on the single-strand ...

...regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces ... expression of a truncated protein. Targeting an exon coding for an essential protein domain could abolish...

...libraries often target 5′ constitutively expressed exons, while activation and repression libraries target...