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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4 results for: EXO

Showing: 1 - 4 of 4 results

  1. Cloning

    Type
    Guide
    ...molecular biology enzymes: 5' exonuclease, polymerase and ligase. 5' exonuclease digests the 5' end of dsDNA...) relies on the 3'-5' exonuclease activity of T4 DNA polymerase. An exonuclease is an enzyme which removes... thus does not need restriction enzymes or an exogenous ligase providing an incredibly quick and easy ...together in one isothermal reaction. First the 5’ exonuclease chews back the 5’ ends of your DNA fragments ...polymerase then closes the gap created by the 5’ exonuclease and finally ligase seals the nicks in the DNA...potential to be completely chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson... DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates “chewed-back” overhangs of 10...

  2. Plan Your Experiment

    Type
    Guide
    ...Cas9 nickase) Single (or dual) gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes...gRNAs commonly target 5′ constitutively expressed exons, which reduces the chances that the targeted region...removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since frameshift... Alternatively, gRNAs can be designed to target exons that code for known essential protein domains. The...

  3. Molecular Biology Reference

    Type
    Guide
    ...antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. When a plasmid ...

  4. CRISPR Guide

    Type
    Guide
    ...libraries often target 5′ constitutively expressed exons, while activation and repression libraries target...
Showing: 1 - 4 of 4 results