We narrowed to 306 results for: SPI;

...pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike protein on the envelope plasmid...2 researchers began pseudotyping the SARS-CoV-2 spike protein into a lower BSL virus like lentivirus (...Crawford et al., 2020). That way, they can study the spike protein’s role in infecting cell lines without using...Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays. Viruses 12:513...

...hierarchy: class to type to subtype to variant. Despite the seemingly complicated steps to arrive at this...largely underutilized compared to Class 2. Class 1 Despite their incredible abundance, Class 1 CRISPR systems...accessory Cas proteins in the Cascade complex. Despite a tenuous grasp on how type III systems function... III-E effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a single protein effector...Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides...

...immune system to fight progressing cancers. It was inspiring to participate in the 2017 Cell Symposium on Cancer...Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed and...Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation... evade the immune system - COX inhibitors like aspirin may give our immune system the boost it needs to...

...mammalian expression vectors containing various spike protein variants are also available. Plasmids are...include proteins like nonstructural proteins 1 to 16, spike proteins, envelope small proteins, and membrane ... length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector...Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana...

...Mark Mellett, a senior researcher at University Hospital Zurich. IL-36 cytokines are a potent inducer of...chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic valve that has two leaflets...study is to understand why women with TS develop bicuspid aortic valve at a rate that is 50-100 times higher...of our research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans...

... coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue...fluorescent proteins, it’s important to note that despite their distinction by name, chromoproteins are actually...

...transduce, you may want to consider spinfection. Spinfection is the process of transducing cells at a concentrated...than the number of plasmids in the library.  Spinfection If you have a large number of cells to transduce...concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary across cell ...

...fate when she pricked her finger on the spindle of a spinning wheel but also a biological transposon system... one patch on the surface, giving the embryo a “spiny and prickly look” that resembled a hedgehog (Nusslein-Volhard...named by a postdoc in Cliff Tabin’s lab who took inspiration from a comic book his daughter was reading. SHH...

...popularity over the last decade. Research by Scott Spicer at the University of Minnesota helps illustrate...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...research is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract...the numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals...

...bearing the "Bunch of Baby Spinach"aptamer, which is a brighter version of Spinach2, an RNA aptamer that fluoresces.... Bunch of Baby Spinach, or BoBS, consists of three tandem copies of the Baby Spinach core embedded in...with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your RNA experiments... - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer, and more - to sequences on a Gaussia ...

... at Harvard Medical School/Brigham and Women’s Hospital (BWH). Based on her experience co-directing Mentor...supported, and can more fully identify with and be inspired by the mentor’s successful example. For evidence...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...the Mentor Circle Program at Brigham and Women's Hospital Visit the International Mentoring Foundation for...

... Confocal Microscopy (Spinning Disk) Parallelized Confocal Microscopy (Spinning Disk) increases the speed...use parallelization to enhance performance. In spinning disk microscopy, a metal disk containing a number...with a parallelized confocal technique such as spinning disk confocal. However, due to the scattering ...

...holidays may be over, but signs of holiday spirit still linger (despite the unseasonably warm weather we had... Miller lab annual #DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to...

...Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church and Collins...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

...upstream of the sequence of interest. 5. Use a silica spin column for purification of the samples you send ...isopropanol precipitation method or using a silica spin column available from several vendors. The precipitation...below), so I would strongly recommend using a silica spin column. They can be pricey, but well worth it. ...

... Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to...CRISPR/Cas9 for targeted genomic deletions We were inspired to produce intrachromosomal deletions based on...and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab studies the...

...and ideas. Maintaining openness and looseness of spirit The second crucial element in harnessing the power...serendipity is maintaining an openness and looseness of spirit. Amidst the rigors of PhD studies and long days...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ...

...expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system. This created... tails still free, and different regions of the spinal cord were illuminated. By looking at groups of ...dissection of a behavioural module in the vertebrate spinal cord." Nature 461.7262 (2009): 407. PubMed PMID...

...publication by Xiuyuan Ou, et al. that characterized the spike (S) protein of SARS-CoV-2 (Ou et al., 2020). The...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...Jin Q, Wang J, Qian Z (2020) Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its...

...students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks to our guest blogger, Mary Tamer...

...tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor...proteins.  To infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric...outreach programs to encourage and embolden the spirit of scientific innovation amongst school children...

...will lead to advances in human health. It was inspiring to hear how the genome project has already had...antibiotic treatment resulting in major problems in hospitals. Dr. Qimron proposes using bacterial phage vectors...sensitive to known antibotics. These might be used on hospital surfaces and in hand sanitizers.  There are many...

...t in immediate use. This will prevent spills, but, if any spills do occur, they should be wiped with 70%...enough space to move around freely without causing spills. Also, keep all liquid containers closed when they...

...product is toxic to cells at high concentrations. Despite their advantages, mammalian expressions systems...provides the highest level of protein expression. Despite their ease of use, it is important to note that... access and purification of a desired protein. Despite being a rather nascent technology, plant cells ...

...delivered to the central nervous system via cerebral spinal fluid. The injection site is the space in and around...minimally invasive. Restraint: anesthesia Location: spinal cord Gauge: 30 Volume: up to 0.01 ml (10 µl) Skill... 26 < 0.05 mL Advanced Intrathecal Anesthesia Spinal cord 30 up to 0.01 mL Expert Intratracheal Anesthesia...

..., emission spectra of certain fluorophores can “spill over” into detectors that are not the primary detector.... For example, in figure 1, the FITC signal is spilling over into the primary detector for PE. In order...compensation has to be applied to “subtract” this spillover and correctly distinguish between markers. Additionally...

...straightforward. When your agrobacterial cultures are ready, spin them down and resuspend them in transformation media...construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains see Weigel... likely you are to get a transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of...

...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....purification kit, you can still reduce the number of spin columns you need to complete your research. Do you....1371/journal.pone.0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...

...histone genes in Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism...so can be used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black... feeding and maintenance that had the audience gasping. Animal husbandry of diverse species is a real ...

...consequences While frustration and anxiety have inspired people to rally for science, others are concerned...science rallies scientists to political action, and inspires politicians and citizens to include science in...state in this map. Finally, the march is meant to inspire scientists to be more involved in their communities...