We narrowed to 755 results for: ELP

...This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help you define... resource budget can help you compare the monetary and empirical value that helps you determine whether...budget, create a start up list.  In this post we’ll help you successfully negotiate a start up award by giving...writing down prices as you create your list. This helps to ensure that all of the resources are accounted... skill sets needed to do your science. This will help you identify the talent pool that you’ll need to...practice of putting together a resource budget will help you focus on your research goals and hopefully remove...applications, etc. If this is the institute that can help you and your research, then it’s worth working within...

...crowdsourcing experimental help. We love it when a researcher reaches out to ask if they can help us create or update...countries that would benefit from a third-party “helper”: a distributor who, among other things, could ...Brazil   In some cases, the distributors broadly helped increase access in the country, and we can see ...with them (Figure 2). In others, the distributor helped solve an issue specific to that country. ‌For example...from scientists in China. They were also able to help with sharing information through the use of popular...promoters, expression types, and more, all of which help a researcher understand if a material is right for...We also introduced guides — practical primers to help users get started with tools like CRISPR, lentivirus...

...media to help my research and my career. Here are three examples of how using social media has helped me or...platforms, and 3) helped you fit social media into your busy schedule. Has social media helped you advance ...ElizabethKenkel) December 5, 2018 How social media has helped me in science It may have taken a bit of FOMO to...learning about new scientific developments and helping scientists is a win-win for me. By using Twitter...the AAV vectors in the paper seemed like it could help one of my lab mate’s with her project. Unfortunately... had something to do with it. Five resources to help you get started with social media as a scientist...challenging to use, it’s hard to know where to begin. To help others start using social media to their professional...

...of advisors who can help us in different ways and in diverse areas. It is helpful to have mentors from...organization. The mentor in your own workplace is able to: Help you identify specific skills you need for your current... present or see your work product in action) Can help you learn the local “ropes” and become an insider...faster A mentor that works elsewhere is able to: Help identify the skills you will need to make a change...mentoring program, consider tapping into it and helping make it work by being an educated mentee. Other.... Tell them how their advice made a difference, helped you make a change for the good or resulted in a...meeting goes well, you can say, “This was really helpful, perhaps you wouldn’t mind doing it again next ...

...When you have time, would you mind helping me with….” vs. “I need help with…now”. Ask for feedback so you...mystery – there are some practices that can really help! Following best trainee practices gives twofold ... you report or talk to on a daily basis who will help you get on your feet with techniques in the lab ...blog, eBooks, and other educational resources to help you get started!) Are there protocols you can familiarize...questions to your training! All of these things will help you get more out of the in-person training component...time. Don’t: Wait to ask questions and/or receive help until you’ve already started a time sensitive experiment...Requests like this are both very normal and very helpful; don’t be afraid to ask! Especially for those new...

...will help your scientific writing across all platforms, from posters to papers.  We hope this helps, and...to use words to describe the image in a way that helps visually impaired readers receive the same information...all of this information to describe the image helpfully. You and your readers are now a team, working ...together to share information. Sometimes it is most helpful to use a lot of jargon and have a technical, detailed...detailed approach. Other times, it is more helpful to use plain language and focus on a broad takeaway...takeaway.  In this image, we thought it would be most helpful to highlight the technical details of collecting...steps for any type of writing. You can use them to help you write an entire manuscript, or you could use...

...offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... useful for finishing your thesis as it is for helping your team reach an important milestone at work....better sense for what problem solving entails will help keep you motivated when you’re faced with yet another...Scientific Support team, much of my day is spent helping to resolve problems, but even so, I wasn’t sure...in the lab.    I like this definition because it helps me see problem solving as a generalizable process...: Identify the problem and gather information to help you understand it. Step Two: Come up with and evaluate...taking a conscious or active approach can be hugely helpful not only for developing a skill but also for being...

...the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process Resources...time. Though I now spend far less time answering help emails and phone calls, I feel compelled to share...ordered something from you and it’s wrong. Can you help us fix it? Please address as soon as possible!” ...begin my responses asking for more specifics. We can help you faster if we get as much information as possible...or a sequencing trace file can go a long way in helping us understand the nature of your concern. Please...directly responsible for the problem. We’re here to help. You've asked your question, and now... One of ...never be painful. Following the tips above should help you get the most out of your interaction.   Resources...

...I’ve learned that some of the technologies that helped us navigate a global pandemic can also be the thing... thing that provides a new perspective that helps to clarify the harder parts of the application process...that advisor’s research interests which can be helpful in creating your application to that program. An... of your program and ask any questions that will help you determine if the program is the right fit. Reaching...make the virtual environment more comfortable and help you to keep yourself organized. By writing down ...you won for your conference presentation. It can help soothe your nerves and make sure you cover everything... off. This question and answer process can also help you to see if the institution is right for you outside...

... create community to help Involve family. If you don’t have local family to help, create “fake” family... running errands. Give help when you have time, and in return you will get help when you need it. On the...other interests and priorities, but many find it helpful to obtain support and ideas from those who seem...reminded of the seemingly simple actions we can take to help ourselves can be an appropriate first step. Check...partner CAN take the kids to the doctor without your help. Your partner is equally responsible and capable...the neighbors and create a network of support to help with elder care, carpooling, cooking on nights when... you can count on for tips and tactics that will help you do your job better. #8 Thou shalt enthusiastically...

..., it can help the hiring manager understand how your specific skills and experience will help you excel..., so we've updated our cover letter blog post to help you write the best one possible!  Should I write...won’t hurt your application, but it can certainly help you stand out.  In some fields, a cover letter is...letter for? A cover letter is a place to provide helpful context about your candidacy, beyond what is on... explaining how your experience and skills would help you do the job as described. Let’s imagine you’...designing it the way you did, and state that it would help you manage a large number of files as part of the...to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good writer? That’...

...? What help did you need? What help did you solicit? When did you first see you needed help? What ...series on Management for scientists. Feedback is help and it should result in authentic assistance for...person's performance or what has been learned, it helps to plan effective next steps. For a formalized feedback...What kept you from getting the help you needed? What could I (manager) have done differently? What Can...to go when giving feedback. The employee is not helped if the feedback session is over and they still ...feel about their performance. Giving honest and helpful feedback gets easier with practice so you must ...

...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...perhaps you have a colleague who seems fun and helpful who you’d like to get to know better. Networking...paper, protocol, or other information that may be helpful or of interest to them. Don’t send a long email...appreciation. Send a thank you note or gift to someone who helped you succeed in any way. Don’t believe people who...of time. People that don’t know you, can’t really help you. The exception is having a mutual acquaintance... discussing non-work topics and interests. Offer help when you can, instead of just asking for favors....be creepy. There is a person on LinkedIn I once helped by answering one simple question so I accepted ...

... to help researchers in creating and sharing knowledge, related to the types of materials we help distribute...favorite content collections is our Help Center, help.addgene.org, where you can easily search articles...from thousands of laboratories that makes up our Help Center, blog, website, social media, videos, newsletters...we may need to reach out to an external expert to help us create accurate content. Regardless of the process...Final touches We try to use figures and images to help illustrate our points and break up walls of text...scientific community, and we can only do that with your help.     Additional Resources on the Addgene Blog ...

...offering advice on areas where you might need some help. Today: Teamwork. My first experience on a successful...time to update how we make and distribute kits, I helped assemble a crack team of Addgenies that includes...nuance of how kits work at Addgene, but with the help of these people and others, we have updated our ...might find that your next paper is going to require help from a biochemist, a geneticist, a microscopist,...ve seen so few in my 20+ year career that I can’t help but feel a bit dubious when I do come across one... to crave. The benefits of a team So why is it helpful, or sometimes even necessary, to build a team in...science any favors by trying to go it alone. The help of fellow lab members and collaborators will not...

...system, however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on... diligently throughout the production process.  Helper-free production Unlike lentivirus, AAV does not... the ability to replicate itself and depends on helper viruses like adenovirus (Ad) or herpes simplex ... on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and VA RNA genes; and...factors and serotype-specific capsid genes.  In this helper-free system, HEK293T cells, expressing Ad E1A and...plasmid, carrying your gene of interest, and the two helper plasmids, commonly called “triple plasmid transfection...can be performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the ...

...need help from another person/team at the company. How do I negotiate with the person/team for help? What...offering advice on areas where you might need some help. Today in our transferable skills guide: Negotiation...problem-solving exercise? Asking questions like the below can help you understand the perspective of all persons involved...have extra time next week (after her deadline) to help you. Her feedback on your manuscript is more important... neutral third party can be a useful strategy to help get unstuck and move closer to an innovative resolution...feel more natural. Creative negotiation skills will help you strengthen relationships with your colleagues...negotiation is a key transferable skill that can help you get more of what you want and can also strengthen...

...lymphocytes (CTLs), natural killer (NK) cells, and helper T (Th) cells. These cells produce inflammatory ...Finally, signals released from the microenvironment help create tolerance beyond direct proximity to the ...dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments for tumor cells spreading...metastasis, block the cell-surface proteins that help escape immuno-surveillance, and curb cytokine and...the tumor microenvironment TGFβ, IL-10, can also help the immune system attack tumors. Attempts have been...popular whole cell therapeutics because they can help start a sequence of immune reactions that aid in...positive effects of the therapy being tested and will help healthcare professional prescribe and identify potential...

...opportunities to work for biotechnology companies and help other researchers use the company’s products successfully...the specificity of this antibody?” You will also help troubleshoot products, give general experimental... should also demonstrate a strong willingness to help others with their problems, a general positive attitude...and an open-minded personality. These traits will help you deal with any incoming requests or problems ...and maybe also the proposed salary range) should help. In smaller companies, technical support is often...replacement products. In order to find good and helpful answers for customers, internal resources and databases...that these skills are highly transferable and will help you enter other roles in project management, consulting...

...abilities, increased my technical know-how, and helped develop my science communication skills. Ultimately...should earn a master? Here are a few questions to help you decide if a master’s might be for you. 1. ...employment, so it’s important to make sure a masters helps you get a job you want. But how do you know if a...capture all of the responsibilities of a role, so it helps to see how people describe their actual work. To...advancement and growth? A master’s is a tool that can help you advance your career, promote career growth, ...prepare you to present your work at conferences, help with manuscript and grant preparation, and lead ...but hopefully the questions in this blog post can help you start to figure out if a master’s is a good ...

...distribute, please send an email to help@addgene.org. Your questions help us expand and improve our resources... researchers we’ve met with have asked if we can help them share and distribute other reagents. Virus ...their own.Harvard researcher Connie Cepko has been helping us with AAV testing and recently had this to say...perform a genome-wide screen, we’ve got viruses to help you out. Of course, lentiviruses are used in experiments...Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector educational...develop it. More communication and sharing will only help research advance. Remember: productivity is infectious...

...rely on the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery...do that we can use the whole science community's help. You could be the next scientist joining in this...(6 feet apart), to fight the new Coronavirus. To help get you started, here are a few key details. Our... might want to be careful with these clones (and help us figure out what is going on there!).  Find the...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can...

... packaging or replication components are called helper plasmids. Below, we’ll breakdown the vector components...required for lentiviral production, they can be helpful for certain applications and aid in ease of use...humans. Cap is a structural capsid protein that helps with virion assembly and particle release from late...genes – E4, E2a, and VA – which are supplied on a helper plasmid and mediate efficient viral replication...and entry Adenovirus is quite large and has more helper plasmid components than the other viruses discussed...viral vectors Addgene has many viral vectors and helper plasmids for all the viruses discussed above (gamma-retrovirus...construct on your own? No problem, we are here to help with resources from AAV variables that matter to...

...My role at Suffolk is to help create new programmatic content as well as help our students succeed whether...person. These interactions could either help you in your career or help them in their career and enhance the...ability to identify and fulfill a need in terms of helping the scientific community manage those plasmids ...international presence would potentially also be very helpful to the scientific community.Paratore: I’ve certainly...auditor. I oversee a department of 20 individuals that help the plan manage risk. My job and my team’s job is...

...applying to the opportunities of their dream.  To help students focus on the application process, and simplify...interested in.     STEAMid is an app that helps you find internships and scholarships in different...page. Finally, as previously mentioned, enlist the help of your network to polish the final draft prior ...doubt, consult the web to find the tools that can help you craft a solid personal statement.  4. Letters...and personal statement along with the request to help your recommenders deliver a superb and detailed ...network of friends, and future colleagues that can help pull one another up while you are climbing the academic...communities to educate parents about resources that can help their children thrive academically. Rose is the ...

... thank some of the people and organizations who helped us reach this milestone. Viral service supporters...Chavez from the Wyss Institute were instrumental in helping us establish consistent, high-quality protocols...supportive Board of Directors at Addgene. Our Board helps keep our focus on assisting scientists and accelerating...and information. Addgene has been successful in helping thousands of sharing scientists from laboratories...ourselves, “How can we do this in a way that most helps scientists and allows us to maintain Addgene’s high... expertise in answering questions on virus and helping provide educational resources on this collection...viral vectors.  How Addgene’s Viral Service will help scientists Good science isn’t the only facet of...

...applications throughout my graduate career, and helped receive funding towards my PhD project. Grant ... in a few days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in.... Using bold, italic, or underline font formats helps these statements stand out from the rest of the ...hired, direct and indirect costs, etc. It may be helpful to ask your advisor to supply a budget form used...Whether the proposal is funded or not, the comments help gauge room for improvement, deliver outsider opinions...experience in communicating and motivating research will help you understand the process and continue to improve...What types of writing feedback do you find most helpful? Have a great grant writing tip? Share it with ...

... over time. Creating an alert for a search term helps automate your search for new papers. An alert lets...follow: Scientists: Following other scientists helps you stay on top of what others are studying and ... support. Monitoring these types of accounts can help you find papers related to a particular topic or...likes, and comments on a tweet about a paper can help you gauge how “hot” a paper is.  Best uses Finding...to a specific window of time for publication. To help you quickly identify papers with greater influence...You can add hashtags to library entries, which can help you organize and search for papers later. Pro tip...suggested related researchers, journals, and concepts to help you expand your search. Results in your feed are...

...shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication... my lab’s protocols and some great co-workers to help me out. Even with these tools, I found myself writing...packaging PEI transfections. It saves me time and helps me figure out if I have enough plasmid to complete...gradient layers, and has some great pointers that while help you master iodixanol AAV purification. If you’re...layers before doing your first purification. This helped me get a feel for layering the iodixanol solutions...two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your larger aliquots...

... this will help you avoid cross-contamination through accidental mixing and will also help you keep good...are some tips for cell culture that will hopefully help you keep a well organized lab and contamination ...light 3-4 times a week for up to half an hour can help keep your hood contamination free, but you should...wipe them all down before you start. This will also help you avoid moving things in and out of the hood while... when putting them into the incubator). It also helps to get familiar with the morphology of the cell ...doing a quick check under the light microscope will help you identify bacterial and yeast contaminants. ...

...meetings more productive for attendees and helping societies help their members get the most out of their...willing to introduce myself to someone new and ask for help. That was NOT something I would have done at my ...Binning Singletons logo. Image: James et al., 2020. Help me stranger - Finding mentors for the meeting Just...attended the meeting multiple times before and would help the Singletons plan for and network at the meeting...meeting. We found our mentors by asking for help with the program on social media. Many of the people who...that the program was largely a success, but also helped us find areas for improvement. We will make a greater...

...situation that presented the challenge at hand and help resolve it through well-informed recommendations...therapeutics. Consultants often work with such teams to help make decisions about R&D investments in either new...treat, and diagnostic advancements. Consultants can help these teams develop communication strategies, or...anticipated commercial success. Or the client might need help with the intricate task of designing a strategic...innovative mobile phone applications and services that help diabetic patients manage doses and timings of their...expectations. Such situations can get stressful and to help manage that, most consulting firms coach and counsel...business schools, and consulting clubs are especially helpful since they both provide up-to-date information ...

...abstract and more. We've also included a feature to help save you from performing any unnecessary cloning...that have used your plasmid of interest, which may help you identify relevant protocols and experimental...Plasmid" list increasing your work's visibility Help us improve our citations lists We are still growing...Plasmid" database - and we always appreciate any help we can get. If you know of an article that used ..."Articles Citing This Plasmid" list. Email us at help@addgene.org with the Plasmid Name, Addgene Plasmid...to the repository, email us and we'd be happy to help. Read More Tips from Addgene Plasmids 101: How...

...courtesy of jorenl/Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well...Disraeli Mentor, Sponsor, Advisor, Boss – who will help me advance my science career? We spend many years...mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive ...mentoring relationships with advisors and others who helped shape their experience. This led to the topic of...micromanage (teaches you to fish, doesn’t fish for you) Helps identify a Mentee’s strengths and make the most ...

...repository can help them share their materials. The new office in London is dedicated to helping them find ... getting them from collaborators was extremely helpful, but with hindsight I wished I had gotten them ...force and as an Addgenie I will do all my best to help them in their tasks. But I won’t be alone. I join...perfect Addgenie and she can’t wait to see how she can help the scientists in Europe with their research.   ... maybe even visit your lab! We look forward to helping you make some serious discoveries.   10 Years ...

...Are you good at asking for help? If you are good at asking for and getting help, size might not be a big... the sections below. Mentoring relationships can help identify your priorities and needs, but sometimes...to run a lab if that is your aspiration? Will you help or at least observe grant writing? Get equipment...of disease, drug properties, etc.)? Will this lab help you head for a career in drug/device development...collaborations Reputation for not being open PI helps lab members determine project "ownership" and author...or postdoc lab. Having a comparison can be very helpful in uncovering strengths and weaknesses of the options...

...they tend to be scattered about the internet. To help you keep your virtual molecular biology toolbox ...some way before you can analyze them. These tools help with that.  DNA sequence reverse complement tool...Cloning ligation reaction calculators These links can help you calculate how much insert and backbone you need...calculators Lab math calculators These calculators can help you with common calculations performed in the lab...iPhone Reagent and Data Repositories These links can help you find experimental and/or validation data on ...blog: Learn how the CRISPR software matchmaker can help you choose the best CRISPR software for your needs...

... grad school? How did these societies help (or continue to help) you grow as a scientist and what benefits...events and summer internship information sessions to help undergraduate students with their applications. ...undergraduates and PhD students so that they can help support each other and provide advice. The society...events, and networking meetups. The society hopes to help young scientists interested in this field excel ...the scientific community. While we are not able to help with every request, we do aim to support events ...

...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save you... also handle the MTA, QC the plasmids, provide helpful resources, and, on the rare occasion things go ...well (or 384-well) plate! How much do repositories help?   Designing precise calculations for any of the...numbers for consumable costs, our depositors have helped us save the scientific community well over $80 ...expect that resources like our AAV Data Hub will help scientists save even more!) If you assume an average...Additional Resources on the Addgene Blog 5 factors to help you choose the right cloning method Find blog posts...

...this article, there are a few principles that will help you resolve most conflicts, even with difficult ...difficult people. The following seven strategies will help you to become more assertive and resolve disagreements...cannot resolve a problem on your own, reach out for help so you can get the support you need. 2) Prepare ... commitments. Assertiveness is a skill that will help you to resolve both personal and professional conflicts...conflicts. At the workplace, these strategies will help you to communicate with confidence earn respect ...difficult person, the above strategies will still be helpful but you will need to be more assertive to get your...