We narrowed to 116 results for: 110
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TypeBlog Post...modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott,...
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Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...methylation-deficient bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for... -
Summer SciComm: Preprints
TypeBlog Post...survey of bioRxiv authors. bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott...preprint server for biology. bioRxiv. https://doi.org/10.1101/833400 Stern BM, O’Shea EK (2019) A proposal for... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R, Dingens...evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (2020) Open... -
Hot Plasmids: Winter 2025
TypeBlog Post...024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803). CLIP, CLAP, SNAP! Self-labeling...bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623. A fast and responsive voltage... -
Getting to Know Expansion Microscopy
TypeBlog Post...Transactions Royal Soc 380:20210110. https://doi.org/10.1098/rsta.2021.0110 Sherry DM, Stiles MA (2022... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-...cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117 Kodama Y, Hu C-D (2012) Bimolecular... -
Lentiviral Vector Uses and Overview
TypeBlog Post.... PubMed PMID: 9765382. PubMed Central PMCID: PMC110254. 3. In-vivo RGB marking and multicolour single-cell.... PubMed PMID: 9733856. PubMed Central PMCID: PMC110156. 10. Integrase-defective lentiviral vectors: progress... -
Hot Plasmids Spring 2024
TypeBlog Post...bioRxiv 2024.03.17.585235; doi: https://doi.org/10.1101/2024.03.17.585235. Improved prime editor PE7...bioRxiv 2023.12.12.571321; doi: https://doi.org/10.1101/2023.12.12.571321. Revolutionizing Gene Therapy... -
Hot Plasmids: Summer 2024
TypeBlog Post...bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499. LEA proteins protect fragile...bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238. Engineered and evolved optimizations... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post... transmission. bioRxiv 2022.02.13.480251. doi: 10.1101/2022.02.13.480251 JEDI-2P voltage sensor The François... living cells. bioRxiv 2022.08.13.503850; doi: 10.1101/2022.08.13.503850. iGECInano NIR calcium reporter... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...2011 Mar 25;286(12):10017-26. doi: 10.1074/jbc.M110.176636. Epub 2011 Feb 1. PMID: 21285356; PMCID: PMC3060453...Med. 2020;20(10):798-805. doi: 10.2174/1566524020666200427110452. PMID: 32338220. Crozat E, Fournes F, ... -
The Pleiades Promoter Project
TypeCollection... Ple171 RAMP3 pEMS1106 EGFP/cre/NLS Ple171 RAMP3 pEMS1410 EGFP/NLS Ple172 RAMP3 pEMS1107 EGFP/cre/NLS ... Ple173 RAMP3 pEMS1108 EGFP/cre/NLS Ple173 RAMP3 pEMS1412 EGFP/NLS Ple174 RAMP3 pEMS1109 EGFP/cre/NLS ... Ple183 S100B pEMS1102 EGFP/cre/NLS Ple183 S100B pEMS1382 EGFP/NLS Ple184 S100B pEMS1103 EGFP/cre/NLS ...Ple47 CX3CR1 pEMS1518 intron-lacZ/NLS Ple48 DBH pEMS1110 EGFP/cre/NLS Ple48 DBH pEMS1359 EGFP/NLS Ple48... EGFP/NLS Ple109 HBEGF pEMS1580 intron-lacZ/NLS Ple110 HBEGF pEMS1255 EGFP/NLS Ple111 HCRT pEMS1051 cre...NLS Ple231 TNNT1 pEMS1435 EGFP/NLS Ple232 TNNT1 pEMS1100 EGFP/cre/NLS Ple232 TNNT1 pEMS1436 EGFP/NLS Ple232...Ple232 TNNT1 pEMS1704 intron-lacZ/NLS Ple233 TNNT1 pEMS1101 EGFP/cre/NLS Ple233 TNNT1 pEMS1437 EGFP/NLS Ple234... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... Crystallization Communications 61.12 (2005): 1100-1102. PubMed PMID: 16511246. PubMed Central PMCID: ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Nature, 2011. 470(7332): p. 105-9. PubMed PMID: 21151107. PubMed Central PMCID: PMC3033971. 18. Huang, ... PubMed PMID: 21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic... -
Immunology Research Plasmids and Resources
TypeCollection...PI3Kbeta, PIK3C1, p110-BETA PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide...Synonyms ADRM1 adhesion regulating molecule 1 ARM1, GP110, MGC29536, Rpn13 AP3B1 adaptor-related protein complex...class I, B AS, HLA-B-7301, HLA-B73, HLAB, HLAC, MGC111087, SPDA1 HLA-C major histocompatibility complex,...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related..., mE10 BLNK B-cell linker BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65 BTK Bruton agammaglobulinemia tyrosine... -
Brain Initiative Collection
TypeCollection...entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated...expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated...expression of dLight1.1 under CAG promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated...expression of dLight1.1 under CAG promoter Lin Tian 111068-AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated...expression of dLight1.2 under synapsin promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated...expression of dLight1.2 under synapsin promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2 To generate Adeno-Associated... -
Neurodegeneration Plasmid Collection
TypeCollection...ALS Sachdev Sidhu 110028 pHH0103_PFN1_Profilin PFN1 GST tac ALS Sachdev Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3...'s Dan Mulvihill 110486 Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-...Huntington's Baoji Xu 110488 pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145...'s Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David Komander 110945 pmTurquoise-parkin...-TDP43 WT] TARDBP CMV ALS Rajat Rohatgi 107840 pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM] TARDBP...TARDBP CMV ALS Rajat Rohatgi 107841 pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD] TARDBP CMV ALS...T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139110 pJ4M_hnRNPA2_FL_D290V HNRNPA2B1 T7 Multisystem ... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection... phosphoproteome. The Rinehart lab synthesized 110,139 unique DNA sequences corresponding to every previously...phosphoproteome. The iSPI resource subdivides the 110,139 human phosphoserine site library ( 188536 ) into...Biol 2023 Aug 8;19(8):e10591. doi: 10.15252/msb.202110591. PubMed (Link opens in a new window) Protocols... -
Gibson Assembly Protocol
TypeProtocol...Isothermal Assembly. bioRxiv . https://doi.org/10.1101/2020.06.14.150979 (Link opens in a new window) ...