...mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine...editing in real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists... Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al....expression to cells expressing eGFP and mCherry. ACE was used to identify cells that have been successfully...and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from the Harris Lab relies on the restoration...for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time..., and luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...

...a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red ...Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...seven or eight amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin... an increase in the neuron’s membrane potential, Ace absorbance increases and results in higher quenching...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019. ...

...specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. 2016. Programmable...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds to...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the crRNA...Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially... development has occurred at a truly astonishing pace, with work directed towards increasing on-target...target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting...

...the ACS career fair is more industry-focused. For another industry-geared option, try the Biospace career...Biology (ASCB) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large...best options are the Naturejobs Career Expo and the ACS career fair, which are larger meetings offering resources...Similar to a conference, job fairs bring you face-to-face with working professionals, with the added benefit...career events page. Biospace holds career events all over the country; sometimes several per month, giving... online career resources Once you’ve identified places you’d like to work, get online and take a look ...know, where they are, and who they know all in one place! This information can be critical to a focused and...

...spheroplasts to release encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check... Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...functionalized so that they adhere to specific surfaces, or they could be decorated with enzymes for use...

.... Created with BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab ...cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021...

...Figure 1: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted to the...Indicators? Electrical signals are integral to many facets of biology. For example, many heart issues stem...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under ...genetically expressed enzyme, typically on the cell surface, which can act on an upstream component, the chemical...Packaging viruses also have various serotypes – the surface proteins that confer affinity for a target – and...

...by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio...52 Parts through Data-optimized Assembly Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi....interested in complex Golden Gate Assembly, a great place to start is the 24-piece lac operon assembly deposited...

...aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology 6.9 (2017): 1710-1721. PubMed PMID...high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420. PubMed PMID...challenging to track intracellularly, but aptamers permit the study of intracellular RNA dynamics. Aptamers...then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul...simultaneously target cellular RNA to specific intracellular locations using Cas9 and track the localization...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ... and both TO1-biotin and TO3-biotin contain an acetate modification that reduces nonspecific binding with...

...shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-57. PubMed PMID: 22894855. PubMed...for the analysis of protein-protein interactions. ACS Chem. Biol. 10, 1797-804. PubMed PMID: 26006698. ...luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release...brighter than RLuc8 (6). Susuki, K, et al. therefore replaced the RLuc8 moieties in their Nano-Lanterns with...biosensors utilizing NanoLuc® luciferase Many intracellular sensors of events like calcium release have ...concentrations of biological molecules. FRET sensors face challenges of photobleaching, autofluorescence, ...Luciferase. CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of ...

...https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...expressed from a separate plasmid in trans, freeing up space in the viral genome so that a gene of interest can...

...doi.org/10.1126/science.1258096 Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR...39–56 . https://doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable... authors were exploring were space and robots. Warping to stars in space ships and the rise of killer ...genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding domain with a single-guide...Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982)... combined the worlds of genetic engineering and space travels. The movie sees the USS Enterprise crew ... Newby GA, Raguram A, Liu DR (2019) Search-and-replace genome editing without double-strand breaks or ...

...Chesterfield et al., 2020     Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/...

...JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH,...bound to the intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...converts a signal received by a GPCR on the cell’s surface into action that can result in the initiation of...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding...

...for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical Biology 2013 Mar 15;8(3):535-42. PubMed ...

...Covalent TMP-Tag Based on Proximity-Induced Reactivity. ACS Chemical Biology, 4(7), 547–556. https://doi.org/...Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://doi.org/... HaloTag for their “ticker tape” method for intracellular recordings, switching between different colored...appearance at the filament tip from (a). c) Intensity traces at the tip of the filament from (a-b), showing ...length (lower). d) Intensity and filament length traces for a different event where CP appears briefly ...ligands! Self-labeling tags can also be used for surface immobilization, purification, or other uses of ...self-labeling protein tags are increasingly popular. Embrace the flexibility and switch things up for your next...

...reproducing useful natural materials.      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio...

...biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and her future...that these enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme...using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria engineered...

...sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133-9. doi: 10.1021...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...FRET-based sensor proteins, particularly for the intracellular imaging of transition metal ions, such as zinc...

... you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE Plasmids: The CRMAGE...Multiplex Transcriptional Repression in E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID...nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested using BsaI, allowing a spacer-repeat...using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. for spacers A, B, and C, you...promoter-gRNA construct, but with different sequences adjacent to the sites. When digested with the appropriate...plasmids are digested, unique overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and...