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Showing: 21 - 40 of 85 results
  1. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...versus the response and use that information to extrapolate the concentration of an unknown sample. For example...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651...
  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...of the sample to that of a standard curve to extrapolate the concentration of antigen in the sample. A... present in the sample and is calculated by extrapolating from the standard curve. Because the primary... present in the sample and is calculated by extrapolating from the standard curve. Scientists often opt...
  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating a true “shock and kill” response. ...on cell viability, cell cycle progression, or apoptosis with Cas9/gRNA expression. A pooled analysis found...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...
  4. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...found that a cytosine base editor (CBE) with rat APOBEC1 causes genome-wide deamination of RNA cytosines...engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of off-target RNA edits...
  5. p53 Pathway

    Type
    Collection
    ...agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase...effects of p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name ...oligomerization. In particular, the loss of p53’s pro-apoptotic effects is especially important to tumorigenesis... below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine...peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, ...protein p53 p53AIP1 Tumor protein p53 regulated apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide...activator inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced death domain protein...
  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Varenicline has been further modified to create ultrapotent PSEMS (uPSEMs) that have drastically increased... A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical...
  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...
  8. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA apoa4-like TAL3023...TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA...
  9. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us how to use dCas9 (with no modifications...
  10. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... as well. This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD...
  11. The Strength of Story Telling

    Type
    Blog Post
    ...it for a while and he eventually calmed down, apologised and admitted he’d been under a lot of pressure...
  12. Hot Plasmids - January 2023

    Type
    Blog Post
    ...  After using mCherry for FACS sorting of the weaponized T-cells (CAR-T cells) - and then delivering them...
Showing: 21 - 40 of 85 results