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...females at an incidence of approximately 1:10,000. It is characterized by apparently normal development through...In females, random X inactivation results in approximately half of cells expressing wild-type MECP2 and...Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in the MECP2 ...

...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...generally ready for shipment within 8 weeks after the approval of the MTA by your institution. Our final step...contact you directly regarding these charges, if applicable....

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...email to [email protected] . After we review and approve the MTA documents, your order will be processed...

...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous... chemical compound and its target protein. The apparent affinity of test compounds is measured by competitive...

...your dissertation. Well, that will probably never happen. Yet with transcription activator-like (TAL) effectors... they still have potential targetable sites approximately every 35bp. Researchers are still determining...arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease...

... the human brain through the development and application of innovative tools enabling large-scale real-time.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Additional Resources To learn more visit:...

...a genomic attP site. So far, their practical application has been limited by low integration efficiency...tetracycline-regulated or other drug-inducible approach may also be used. Split proteins: The recombinase...and cell death. It is important to incorporate appropriate controls such as Cre-only lines to account for...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...Michael Davidson 54571 54856 mT-Sapphire-pBAD tdTomato-pBAD mT-Sapphire (donor) tdTomato (acceptor) FRET...BiFC studies. ID Plasmid Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...

...insertions — meaning they may not be suitable for applications where orientation of the insert is important... TnsC, and TniQ). Unlike type I, type V-K CASTs appear to exhibit almost unidirectional insertions, but...

...development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets... standard desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided...

...family of transporters in the human genome with approximately 450 identified members. Many SLCs have mutations... benefit of basic academic research as well as applied research in small biotech and pharmaceutical companies...

...single round of viral entry and infection. This approach allows researchers to safely study mechanisms ...for SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the search bar to find...

... FREE science lab apps that have been educational and great for lab life. These apps prevent you from ...spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find an app that...I came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics... Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains...Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows...of the many apps available to help researchers in the lab. If you have a favorite lab app, please share...by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article are those of the guest ...

...cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...

... Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory...Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent...