...Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9...Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing the CRISPR-...

...MG1655 and its derivatives DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more ... two individual isolates, the K-12 strain and the B strain. K-12 was isolated from a patient in 1920 and...likely isolated in 1918 but was first referred to as “B strain” in 1942. The BL21 strain and derivatives are... are the most common examples of the E. coli B strain. Common E. coli strains used in the lab Most of...HB101 Streptomycin Hybrid of E. coli K12 and E. coli B (mostly K12, though), common lab strain for cloning...cloning and storage strain. F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 ...sequences with the potential to recombine.  F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697...

...Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U., Schwanke, M., Office, E., Schneider...doi: 10.1242/dev.199399. PMID: 34143204 Goodson, N. B., Kaufman, M. A., Park, K. U., & Brzezinski, J. A....modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...

...expression in hematopoietic stem cells, T cells, and B cells is low. Infection of these cells with VSV-G ...//doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset...in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars were less stable than viruses...express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the envelope and are targets ...targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated ..., and spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–...

...disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency...Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal ...

...region of interest (deletions or small insertions) or (B) span the genome-insert boundary (insertions only)... 11315. PMID: 26066060 Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J.... Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...

...a polypeptide chain, except if their names have a B, J, X, or Z. Some alternative, similar apps: Findings...restriction site to a primer, you only need to skim the alphabet on the side of the screen to find the sequence...

... and the B strain. K-12 has led to the common lab strains MG1655 and its derivatives DH5alpha and DH10b...DH10b (also known as TOP10) among others, while the B strain gave rise to BL21 and its derivatives. We've...Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB Survival Invitrogen...lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697...Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin...Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A, G, or T H A, C, or T K G or T M A ...lacY1 proA2 rpsL20(StrR) xyl5 lambda- leu mtl1 DH5alpha Invitrogen F- Phi80lacZDeltaM15 Delta(lacZYA-argF...

...synthetic GABA(B) receptor ligands (GABA-Snifit) A fluorescent sensor for GABA and synthetic GABA(B) receptor...Nagai Temperature Fluorescent temperature indicator B-gTEMP Intracellular Heat Transfer and Thermal Property...Acid Linkers between a Fluorescent Protein and PDE5alpha. ACS Sens. 2017 Jan 27;2(1):46-51. Tetsuya Kitaguchi...

...region of interest (deletions or small insertions) or (B) span the genome-insert boundary (insertions only)... 11315. PMID: 26066060 Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J.... Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...

...Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment...then be tracked along the secretory pathway (Figure B). The RUSH system has already been used to study transport...

...cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi none S. pyogenes Blast...Lentiviral BsmBI yes, cut S. pyogenes Jacks pU6-sgRNA EF1Alpha-puro-T2A-BFP 60955 Mammalian/Lentiviral none S...