We narrowed to 56 results for: Eomes

1. Plasmids 101: Gene, enhancer, and promoter trapping

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   Blog Post

   ...what remains to be discovered in the eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi....

2. Sleeping Beauty Awakens for Genome Engineering

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   ...with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein...

3. Plasmids 101: Antibiotic Resistance Genes

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   ... cell wall synthesis Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal...

4. Hot Plasmids - May 2022

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   ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and whole organisms...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ... MCS for cloning X gene, an SV40 promoter, and Neomycin selection.   Table 1: Gamma-retrovirus glossary...

6. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   .... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...

7. Visualizing Protein Turnover In Situ

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   Blog Post

   ...visualization of the whole de novo synthesized proteome. However, FUNCAT alone cannot be used to determine...

8. Working with Nuclear Receptors

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   .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read...

10. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... the Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more...

11. Isolating Genomic Regions of Interest with the CRISPR System

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    ...binding partners at numerous genomic loci through proteomics and the chromosome conformation capture (3C) ...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...Merabet S (2018) Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions...

13. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce cell death...

14. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset based on...

15. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...

16. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them. ...

17. Plasmids 101: Expressing shRNAs for RNA Interference

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    .../10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). ...

18. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635...

19. Evolution of Lab Techniques

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    Blog Post

    ...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...

20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting...