We narrowed to 57 results for: Eomes

1. Sleeping Beauty Awakens for Genome Engineering

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   ...with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein...

2. Plasmids 101: Antibiotic Resistance Genes

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   ... cell wall synthesis Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal...

3. Hot Plasmids - May 2022

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   ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and whole organisms...

4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ... MCS for cloning X gene, an SV40 promoter, and Neomycin selection.   Table 1: Gamma-retrovirus glossary...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   .... It also gives cells antibiotic resistance to neomycin so that you can select the population of cells...

6. Rinehart Lab Phosphoprotein Reagents

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   ...positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics . Nature Methods ...(V70) (DOCX, 551 KB) . The synthetic human phosphoproteome The Rinehart lab synthesized 110,139 unique...anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference spectra or...(2022) is adapted from the synthetic human phosphoproteome. It subdivides the 110,139 human phosphoserine...Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent... J. (2018). Kinase substrate profiling using a proteome-wide Serine-Oriented human Peptide library . Biochemistry...

7. Visualizing Protein Turnover In Situ

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   ...visualization of the whole de novo synthesized proteome. However, FUNCAT alone cannot be used to determine...

8. Working with Nuclear Receptors

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   .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read...

10. Light Sheet Fluorescence Microscopy

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    ... the Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more...

11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...Merabet S (2018) Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...bacteria.   Zeocin  Zeocin is a member of the bleomycin family of antibiotics, which induce cell death...

13. Isolating Genomic Regions of Interest with the CRISPR System

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    ...binding partners at numerous genomic loci through proteomics and the chromosome conformation capture (3C) ...

14. Tips for Screening with Yeast Two Hybrid Systems

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    ...interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset based on...

15. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...

16. Plasmids 101: Shuttle Vectors

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    ...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them. ...

17. Plasmids 101: Expressing shRNAs for RNA Interference

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    .../10.1093/nar/gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). ...

18. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635...

19. Evolution of Lab Techniques

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    ...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...

20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting...